ArticleNature microbiology2016
Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales.
Article in Nature microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
90 citing papers in PubMed.
- Geochemistry shapes microbial diversity and selected functional traits in flowback and produced waters from hydraulically fractured formations.FEMS microbiology ecology · 2026Article
- Proteomic stress response by a novel methanogen enriched from the Great Salt Lake.Microbiology spectrum · 2026Article
- Ecological plasticity ofMicrobiology spectrum · 2026Article
- Metabolomics and Human Health: Progress, Insights, Challenges and the Concept of a Healthy Metabolome.Biomolecules · 2026Review
- Phage Frontiers: Genomic and Functional Profiling of Novel Virulent Agents Targeting FoodborneBiology · 2026Article
- Subsurface microbiology and the pressing societal need to support future exploration.FEMS microbiology ecology · 2025Review
- Microbial Community Composition and Function in Jiangsu Oil Reservoir Cores, China.Environmental microbiology reports · 2025Article
- Microbial Considerations for the Permanent Geological Storage of COEnvironmental microbiology · 2025Review
- Polymer biodegradation byApplied and environmental microbiology · 2025Article
- Pathways for Potential Exposure to Onshore Oil and Gas Wastewater: What We Need to Know to Protect Human Health.GeoHealth · 2025Review
- Virolithopanspermia: Might viruses be transported in rocks through space?PLoS pathogens · 2025Article
- Anthropogenic impacts on the terrestrial subsurface biosphere.Nature reviews. Microbiology · 2025Review
- Spatial and temporal groundwater biogeochemical variability help inform subsurface connectivity within a high-altitude Alpine catchment (Riale di Ronco, Switzerland).Frontiers in microbiology · 2025Article
- Inhibition of Microbial Growth and Biofilm Formation in Pure and Mixed Bacterial Samples.Microorganisms · 2024Article
- Illegal dumping of oil and gas wastewater alters arid soil microbial communities.Applied and environmental microbiology · 2024Article
- Comparative single-cell genomics of Atribacterota JS1 in the Japan Trench hadal sedimentary biosphere.mSphere · 2024Article
- Changes in environmental and engineered conditions alter the plasma membrane lipidome of fractured shale bacteria.Microbiology spectrum · 2024Article
- Microbial ecology of the deep terrestrial subsurface.The ISME journal · 2024Review
- Metagenome-assembled genomes provide insight into the metabolic potential during early production of Hydraulic Fracturing Test Site 2 in the Delaware Basin.Frontiers in microbiology · 2024Article
- Deep terrestrial indigenous microbial community dominated byCommunications earth & environment · 2024Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydraulic fracturing is the industry standard for extracting hydrocarbons from shale formations. Attention has been paid to the economic benefits and environmental impacts of this process, yet the biogeochemical changes induced in the deep subsurface are poorly understood. Recent single-gene investigations revealed that halotolerant microbial communities were enriched after hydraulic fracturing. Here, the reconstruction of 31 unique genomes coupled to metabolite data from the Marcellus and Utica shales revealed that many of the persisting organisms play roles in methylamine cycling, ultimately supporting methanogenesis in the deep biosphere. Fermentation of injected chemical additives also sustains long-term microbial persistence, while thiosulfate reduction could produce sulfide, contributing to reservoir souring and infrastructure corrosion. Extensive links between viruses and microbial hosts demonstrate active viral predation, which may contribute to the release of labile cellular constituents into the extracellular environment. Our analyses show that hydraulic fracturing provides the organismal and chemical inputs for colonization and persistence in the deep terrestrial subsurface.
Identifiers
27595198What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.