Evidence map›Paper›PMID 27595149›Full record

ArticleInternational journal of legal medicine2017

Permanganate-assisted removal of PCR inhibitors during the DNA Chelex extraction from stained denim samples.

Sorina Pîrlea, Mihaela Puiu, Adina Răducan, Dumitru Oancea

Abstract read
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Article in International journal of legal medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sorina PîrleaNational Forensic Science Institute, Şoseaua Ştefan cel Mare 13-15, sect. 2, 020123, Bucharest, Romania.
Mihaela PuiuDepartment of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, Bucharest, 030018, Romania.
Adina RăducanDepartment of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, Bucharest, 030018, Romania. adina.raducan@g.unibuc.ro.
Dumitru OanceaDepartment of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4-12, Bucharest, 030018, Romania.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, it was demonstrated that the DNA Chelex extraction combined with the permanganate assisted-oxidation is highly efficient in removing the PCR inhibitors often found in clothing materials, such as phthalocyanine. The extraction assays were conducted in saliva, blood and epithelial cells samples mixed with three oxidation-resistant dye copper(II) α-phthalocyanine, copper(II) β-phthalocyanine and tetrasulfonated copper(II) β-phthalocyanine. After DNA amplification, all samples were able to provide full DNA profiles. The permanganate/Chelex system was tested further on denim-stained samples and displayed the same ability to remove the PCR inhibitors from the commercial textile materials.

Indexed as

Chelating AgentsManganese CompoundsOxidesBlood Chemical AnalysisClothingDNADNA FingerprintingEpithelial CellsHumansIndolesOrganometallic CompoundsOxidantsPolymerase Chain ReactionPolystyrenesPolyvinylsSalivaChelating Agentschelexcopper phthalocyanineDNAIndolesManganese CompoundsOrganometallic CompoundsOxidantsOxidespermanganic acidPolystyrenesPolyvinylstetrasulfophthalocyanineChelexDNA amplificationPhthalocyaninePolymerase chain reaction

Identifiers

PMID27595149

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.