ArticleBioMed research international2016
Is Hydrogen Peroxide a Suitable Apoptosis Inducer for All Cell Types?
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Intratumoral pro-oxidants promote cancer immunotherapy by recruiting and reprogramming neutrophils to eliminate tumors.Cancer immunology, immunotherapy : CII · 2023Pooled it
- Article
- Spatial Patterning of Modular Gelatin-Peroxide Microspheres in Melt-Electrowritten Scaffolds Provides Controlled Oxygen Generation and Mitigates Hypoxia and Cytotoxicity.Advanced healthcare materials · 2026Article
- Cell-Derived Nanocarriers of Apoptotic Bodies with an Antimicrobial Peptide for Targeting IntracellularACS applied bio materials · 2025Article
- Phosphatidylserine-mediated uptake of extracellular vesicles by hepatocytes ameliorates liver ischemia-reperfusion injury.Apoptosis : an international journal on programmed cell death · 2025Article
- Biomimetic composite gelatin methacryloyl hydrogels for improving survival and osteogenesis of human adipose-derived stem cells in 3D microenvironment.Materials today. Bio · 2024Article
- SMYD5 is a regulator of the mild hypothermia response.Cell reports · 2024Article
- SMYD5 is a regulator of the mild hypothermia response.bioRxiv : the preprint server for biology · 2024Article
- Sperm freezing damage: the role of regulated cell death.Cell death discovery · 2024Review
- Finding a Direct Method for a Dynamic Process: The DD (Direct and Dynamic) Cell-Tox Method.International journal of molecular sciences · 2024Article
- Novel Peptide-Based Fluorescent Probe for Simultaneous Sensing of Chymotrypsin and Hydrogen Peroxide.ACS omega · 2024Article
- Cellular Pre-Adaptation to the High OAntioxidants (Basel, Switzerland) · 2024Article
- Article
- A single chemotherapy administration induces muscle atrophy, mitochondrial alterations and apoptosis in breast cancer patients.Journal of cachexia, sarcopenia and muscle · 2024Article
- WNT7A promotes tumorigenesis of head and neck squamous cell carcinoma via activating FZD7/JAK1/STAT3 signaling.International journal of oral science · 2024Article
- Oxygen generating biomaterials at the forefront of regenerative medicine: advances in bone regeneration.Frontiers in bioengineering and biotechnology · 2024Review
- Development of a composite hydrogel incorporating anti-inflammatory and osteoinductive nanoparticles for effective bone regeneration.Biomaterials research · 2023Article
- Mitochondria-targeting EGCG derivatives protect H9c2 cardiomyocytes from HRSC advances · 2023Article
- Article
- Synthesis of Bis-Chalcones and Evaluation of Its Effect on Peroxide-Induced Cell Death and Lipopolysaccharide-Induced Cytokine Production.Molecules (Basel, Switzerland) · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hydrogen peroxide is currently the most widely used apoptosis inducer due to its broad cytotoxic efficacy against nearly all cell types. However, equivalent cytotoxicity is achieved over a wide range of doses, although the reasons for this differential sensitivity are not always clear. In this study, three kinds of cells, the 293T cell line, primary fibroblasts, and terminally differentiated myocardial cells, were treated with a wide range of H2O2 doses. Times to apoptosis initiation and end were measured cytochemically and the changes in expression of caspase-9, P53, NF-κB, and RIP were determined by RT-PCR. The 293T cell line was the most sensitive to H2O2, undergoing necroptosis and/or apoptosis at all concentrations from 0.1 to 1.6 mM. At > 0.4 mM, H2O2 also caused necroptosis in primary cells. At < 0.4 mM, however, primary cells exhibited classic signs of apoptosis, although they tended to survive for 36 hours in < 0.2 mM H2O2. Thus, H2O2 is a broadly effective apoptosis inducer, but the dose range differs by cell type. For cell lines, a low dose is required and the exposure time must be reduced compared to primary cells to avoid cell death primarily by necroptosis or necrosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.