ArticleBiotechnology for biofuels2016
A novel strain of Yarrowia lipolytica as a platform for value-added product synthesis from glycerol.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 93 citations in OpenAlex.
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- The Effective Biodegradation of Poly(ε-caprolactone) by Engineered YeastInternational journal of molecular sciences · 2026Article
- Efficient production of astaxanthin inSynthetic and systems biotechnology · 2025Article
- Synergistic effect of transporter and pathway engineering on the key performance indicators of erythritol synthesis by the yeastApplied and environmental microbiology · 2025Article
- Glycerol bioconversion to biofuel and value-added products by yeasts.FEMS yeast research · 2025Review
- Review
- Research progress on biosynthesis of erythritol and multi-dimensional optimization of production strategies.World journal of microbiology & biotechnology · 2024Review
- Application of NewInternational journal of molecular sciences · 2024Article
- Iterative gene integration mediated by 26S rDNA and non-homologous end joining for the efficient production of lycopene in Yarrowia lipolytica.Bioresources and bioprocessing · 2023Article
- Recent Advances in the Biosynthesis of Natural Sugar Substitutes in Yeast.Journal of fungi (Basel, Switzerland) · 2023Review
- Mutation in yl-HOG1 represses the filament-to-yeast transition in the dimorphic yeast Yarrowia lipolytica.Microbial cell factories · 2023Article
- In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica.Scientific reports · 2023Article
- Effect of Nitrogen Concentration on the Biosynthesis of Citric Acid, Protein, and Lipids in the YeastBiomolecules · 2022Article
- Enhancing erythritol production from crude glycerol in a wild-typeFrontiers in microbiology · 2022Article
- The microbial production of kynurenic acid usingFrontiers in bioengineering and biotechnology · 2022Article
- The Overexpression ofMolecules (Basel, Switzerland) · 2021Article
- Metabolic engineering of the oleaginous yeast Yarrowia lipolytica PO1f for production of erythritol from glycerol.Biotechnology for biofuels · 2021Article
- Application of a New Engineered Strain ofInternational journal of molecular sciences · 2021Article
- Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide resources: review on starch, cellulose, xylan, and inulin.Applied microbiology and biotechnology · 2021Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIncreasing interest of non-conventional yeasts has been observed for many years due to their biochemical characteristics and potential applications. Well-studied, oleaginous yeast Y. lipolytica is an attractive host for converting a low-cost glycerol, into value-added products such as erythritol (sweetener) or citric acid. Glycerol is an important renewable feedstock and is the main co-product of biodiesel production, which is nowadays applied on a large commercial scale. To this end, we engineered the yeast Y. lipolytica to increase the productivity of this strain.
resultsIn this light, we enhanced glycerol assimilation by over-expression of the YALI0F00484g gene encoding glycerol kinase (GK) and gene YALI0B02948g encoding glycerol-3-P dehydrogenase (GDH). The modified strains have been tested for glycerol consumption rate and erythritol and citric acid synthesis under various conditions. Here, we show that the overexpression of GK and GDH, increased glycerol consumption resulting in rapid erythritol and citric acid synthesis. Next, we combined the two genes in the tandem gene construct for the simultaneous co-expression of GK and GDH, which further increased the desired product synthesis. The glycerol consumption was explored in a 5-L bioreactor and the engineered strains were able to utilize 150 g/L glycerol within 44-48 hours. The erythritol productivity for GK overexpression and co-expression of GK and DGH was 24 and 35 %, respectively, over the control strain. Moreover, we established conditions for the production of citric acid at pH 3.0, the engineered strains increased citric acid production 14-fold over the control.
conclusionThis work demonstrates the excellent capacity of the engineered strains as a starting platform for further modification for broad-range value-added product biosynthesis from glycerol. This study presents the highest reported titer citric acid at low pH to date. The process parameters such as productivity and yield of erythritol and citric acid were significantly elevated, what is valuable for industrial applications.
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