Evidence map›Paper›PMID 27594856›Full record

ArticleFrontiers in immunology2016

Efficient and Non-Toxic Biological Response Carrier Delivering TNF-α shRNA for Gene Silencing in a Murine Model of Rheumatoid Arthritis.

Jialin Song, Yinghui Chen, Shichao Jiang, Kejia Yang, Xiaoming Li, Xiaotian Zhao, Yuanming Ouyang, Cunyi Fan, Weien Yuan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

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  7. Applications of RNA interference in the treatment of arthritis.Translational research : the journal of laboratory and clinical medicine · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jialin SongShanghai Jiao Tong University Affiliated Sixth People's Hospital , Shanghai , China.
Yinghui ChenDepartment of Neurology, Jinshan Hospital, Fudan University , Shanghai , China.
Shichao JiangDepartment of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University , Jinan, Shandong , China.
Kejia YangSchool of Pharmacy, Shanghai JiaoTong University , Shanghai , China.
Xiaoming LiSchool of Pharmacy, Shanghai JiaoTong University , Shanghai , China.
Xiaotian ZhaoSchool of Pharmacy, Shanghai JiaoTong University , Shanghai , China.
Yuanming OuyangShanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; Shanghai Sixth People's Hospital East Campus, Shanghai University of Medicine and Health, Shanghai, China.
Cunyi FanShanghai Jiao Tong University Affiliated Sixth People's Hospital , Shanghai , China.
Weien YuanSchool of Pharmacy, Shanghai JiaoTong University , Shanghai , China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Small interfering RNA (siRNA) is an effective and specific method for silencing genes. However, an efficient and non-toxic carrier is needed to deliver the siRNA into the target cells. Tumor necrosis factor α (TNF-α) plays a central role in the occurrence and progression of rheumatoid arthritis (RA). In this study, we pre-synthetized a degradable cationic polymer (PDAPEI) from 2,6-pyridinedicarboxaldehyde and low-molecular-weight polyethyleneimine (PEI, Mw = 1.8 kDa) as a gene vector for the delivery of TNF-α shRNA. The PDAPEI/pDNA complex showed a suitable particle size and stable zeta potential for transfection. In vitro study of the PDAPEI/pDNA complex revealed a lower cytotoxicity and higher transfection efficiency when transfecting TNF-α shRNA to macrophages by significantly down-regulating the expression of TNF-α. Moreover, the complex was extremely efficient in decreasing the severity of arthritis in mice with collagen-induced arthritis. PDAPEI delivered TNF-α shRNA has great potential in the treatment of RA.

Indexed as

PDAPEIrheumatoid arthritisRNA interferenceTNF-α

Identifiers

PMID27594856
PMCPMC4990551

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.