ArticleSensors and actuators. B, Chemical2016
A microfluidic platform for trapping, releasing and super-resolution imaging of single cells.
Article in Sensors and actuators. B, Chemical, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 61 citations in OpenAlex.
- Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Modular microfluidics for life sciences.Journal of nanobiotechnology · 2023Review
- Trapping of a Single Microparticle Using AC Dielectrophoresis Forces in a Microfluidic Chip.Micromachines · 2023Article
- Advances in Single-Cell Printing.Micromachines · 2022Review
- Microfluidics applications for high-throughput single cell sequencing.Journal of nanobiotechnology · 2021Review
- μCB-seq: microfluidic cell barcoding and sequencing for high-resolution imaging and sequencing of single cells.Lab on a chip · 2020Article
- Microfluidic systems for hydrodynamic trapping of cells and clusters.Biomicrofluidics · 2020Review
- Photoactivated Selective Release of Droplets from Microwell Arrays.ACS applied materials & interfaces · 2020Article
- Sheathless Shape-Based Separation ofMicromachines · 2019Article
- Novel PDMS-Based Sensor System for MPWM Measurements of Picoliter Volumes in Microfluidic Devices.Sensors (Basel, Switzerland) · 2019Article
- Single-cell patterning technology for biological applications.Biomicrofluidics · 2019Review
- Sheathless High-Throughput Circulating Tumor Cell Separation Using Viscoelastic non-Newtonian Fluid.Micromachines · 2019Article
- Efficient Low Shear Flow-based Trapping of Biological Entities.Scientific reports · 2019Article
- Luminescent nanomaterials for droplet tracking in a microfluidic trapping array.Analytical and bioanalytical chemistry · 2019Article
- Integrating Immunology and Microfluidics for Single Immune Cell Analysis.Frontiers in immunology · 2018Review
- Reconfigurable microfluidic device with discretized sidewall.Biomicrofluidics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
A multi-layer device, combining hydrodynamic trapping with microfluidic valving techniques, has been developed for on-chip manipulation and imaging of single cells and particles. Such a device contains a flow layer with trapping channels to capture single particles or cells and a control layer with valve channels to selectively control the trap and release processes. Particles and cells have been successfully trapped and released using the proposed device. The device enables the trapping of single particles with a trapping efficiency of greater than 95%, and allows for single particles and cells to be trapped, released and manipulated by simply controlling corresponding valves. Moreover, the trap and release processes are found to be compatible with biological samples like cells. Our device allows stable immobilisation of large numbers of single cells in a few minutes, significantly easing the experiment setup for single-cell characterisation and offering a stable platform for both single-molecule and super-resolution imaging. Proof-of-concept super- resolution imaging experiments with mouse embryonic stem cells (mESCs) have been conducted by exploiting super-resolution photoactivated localisation microscopy (PALM). Cells and nuclei were stably trapped and imaged. Centromeres of ∼200 nm size could be identified with a localisation precision of <15 nm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.