Evidence map›Paper›PMID 27592105›Full record

ArticleCurrent microbiology2016

Characterization of a Rolling-Circle Replication Plasmid pM411 from Lactobacillus plantarum 1-3.

Xvchuan Ma, Jinhua Li, Yao Xiong, Zhengyuan Zhai, Fazheng Ren, Yanling Hao

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Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xvchuan MaKey Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
Jinhua LiKey Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
Yao XiongKey Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
Zhengyuan ZhaiKey Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, Beijing, 100083, People's Republic of China.
Fazheng RenThe Innovation Centre of Food Nutrition and Human Health (Beijing), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China.
Yanling HaoThe Innovation Centre of Food Nutrition and Human Health (Beijing), College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People's Republic of China. haoyl@cau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A cryptic plasmid pM411 isolated from Lactobacillus plantarum 1-3 consisted of a 2303-bp circular molecule with a G + C content 32.96 %. Sequence analysis of pM411 revealed four putative open reading frames (ORFs). ORF1 shared 99 and 94 % similarities, respectively, with the Rep proteins of plasmids pLC2 and pYC2, which belong to the rolling-circle replication pMV158 family. A typical pMV158 family double-strand origin (dso) and a putative single-strand origin (sso) located upstream of the rep gene. Southern hybridization confirmed the presence of single-strand DNA (ssDNA) intermediates, suggesting that pM411 belongs to the RCR pMV158 family. Sequence homology analysis indicated that the sso belongs to the ssoW family. Furthermore, the relative copy number of pM411 was about 88 copies in each cell by real-time PCR.

Indexed as

Bacterial ProteinsBase CompositionBase SequenceDNA, BacterialDNA ReplicationLactiplantibacillus plantarumMolecular Sequence DataOpen Reading FramesPlasmidsBacterial ProteinsDNA, Bacterial

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.