Evidence map›Paper›PMID 27591121›Full record

ArticleMolecular and cellular endocrinology2016

Transplantation of alginate-encapsulated seminiferous tubules and interstitial tissue into adult rats: Leydig stem cell differentiation in vivo?

Haolin Chen, Shiying Jin, Shengsong Huang, Janet Folmer, June Liu, Renshan Ge, Barry R Zirkin

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Haolin ChenCenter for Scientific Research, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China; Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Shiying JinDepartment of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Shengsong HuangDepartment of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA; Department of Urology, Tongji Hospital, Tongji University School of Medicine, Putuo, Shanghai 200065, China.
Janet FolmerDepartment of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
June LiuDepartment of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Renshan GeCenter for Scientific Research, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Barry R ZirkinDepartment of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
Johns Hopkins University · USTongji University · CNWenzhou Medical University · CN

Funding

Aging and Leydig Cell FunctionR37AG021092 · NIA · JOHNS HOPKINS UNIVERSITY · PI ZIRKIN, BARRY R · 2006 to 2015
$4.0M
AGING AND LEYDIG CELL FUNCTIONR01AG021092 · NIA · JOHNS HOPKINS UNIVERSITY · PI ZIRKIN, BARRY R · 2002 to 2019
$3.1M
Hypogonadism in Men: Is It Reversible?R21AG051259 · NIA · JOHNS HOPKINS UNIVERSITY · PI ZIRKIN, BARRY R · 2016 to 2017
$446k
NIA NIH HHS R01 AG021092NIA NIH HHS R21 AG051259NIA NIH HHS R37 AG021092
6 · The paper itself

Abstract

In vivo and in vitro studies were conducted to determine whether testosterone-producing Leydig cells are able to develop from cells associated with rat seminiferous tubules, interstitium, or both. Adult rat seminiferous tubules and interstitium were isolated, encapsulated separately in alginate, and implanted subcutaneously into castrated rats. With implanted tubules, serum testosterone increased through two months. Tubules removed from the implanted rats and incubated with LH produced testosterone, and cells on the tubule surfaces expressed steroidogenic enzymes. With implanted interstitial tissue, serum levels of testosterone remained undetectable. However, co-culture of interstitium plus tubules in vitro resulted in the formation of Leydig cells by both compartments. These results indicate that seminiferous tubules contain both cellular and paracrine factors necessary for the differentiation of Leydig cells, and that the interstitial compartment contains precursor cells capable of forming testosterone-producing Leydig cells but requires stimulation by paracrine factors from the seminiferous tubules to do so.

Indexed as

Cell DifferentiationAlginatesAnimalsGlucuronic AcidHexuronic AcidsLeydig CellsMaleRats, Sprague-DawleySeminiferous TubulesStem CellsTestosteroneAlginatesGlucuronic AcidHexuronic AcidsTestosteroneAlginateLeydig cellsStem cellsTestosteroneTransplantation

Identifiers

PMID27591121
PMCPMC5050555
OpenAlexW2508474019

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.