ArticleMethods in molecular biology (Clifton, N.J.)2016
Directed Endothelial Progenitor Differentiation from Human Pluripotent Stem Cells Via Wnt Activation Under Defined Conditions.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026Review
- Gas vesicle-expressing human pluripotent stem cells enable multimodal ultrasound and optical coherence tomographic imaging.BMC biotechnology · 2026Article
- Notch signaling activation reduces vesicular endocytosis in human pluripotent stem cell-derived CNS-like endothelial cells.Fluids and barriers of the CNS · 2026Article
- Ultrasound Control of Gene Expression in Human iPSCs via Heat Shock Promoters.Biotechnology and bioengineering · 2025Article
- Hypoimmunogenic hPSC-derived cardiac organoids for immune evasion and heart repair.bioRxiv : the preprint server for biology · 2025Article
- Decellularized heart extracellular matrix alleviates activation of hiPSC-derived cardiac fibroblasts.Bioactive materials · 2024Article
- Early chromosome condensation by XIST builds A-repeat RNA density that facilitates gene silencing.Cell reports · 2023Article
- High-efficient serum-free differentiation of endothelial cells from human iPS cells.Stem cell research & therapy · 2022Article
- Article
- Differentiation of human pluripotent stem cells to brain microvascular endothelial cell-like cells suitable to study immune cell interactions.STAR protocols · 2021Article
- Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells.Journal of visualized experiments : JoVE · 2021Article
- Advancing human induced pluripotent stem cell-derived blood-brain barrier models for studying immune cell interactions.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors.Journal of visualized experiments : JoVE · 2019Article
- Quantifying the Vasculogenic Potential of Induced Pluripotent Stem Cell-Derived Endothelial Progenitors in Collagen Hydrogels.Tissue engineering. Part A · 2019Article
- Bioactive Molecules for Skin Repair and Regeneration: Progress and Perspectives.Stem cells international · 2019Review
- Directed differentiation and long-term maintenance of epicardial cells derived from human pluripotent stem cells under fully defined conditions.Nature protocols · 2017Article
- Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells.Bioengineering & translational medicine · 2017Article
- Article
- Long-term self-renewing human epicardial cells generated from pluripotent stem cells under defined xeno-free conditions.Nature biomedical engineering · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Efficient derivation of endothelial cells and their progenitors from human pluripotent stem cells (hPSCs) can facilitate studies of human vascular development, disease modeling, drug discovery, and cell-based therapy. Here we provide a detailed protocol for directing hPSCs to functional endothelial cells and their progenitors in a completely defined, growth factor- and serum-free system by temporal modulation of Wnt/β-catenin signaling via small molecules. We demonstrate a 10-day, two-stage process that recapitulates endothelial cell development, in which hPSCs first differentiate to endothelial progenitors that then generate functional endothelial cells and smooth muscle cells. Methods to characterize endothelial cell identity and function are also described.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.