Evidence map›Paper›PMID 27590116›Full record

ArticleExtremophiles : life under extreme conditions2016

Characterization of two β-decarboxylating dehydrogenases from Sulfolobus acidocaldarius.

Kento Takahashi, Fumika Nakanishi, Takeo Tomita, Nagisa Akiyama, Kerstin Lassak, Sonja-Verena Albers, Tomohisa Kuzuyama, Makoto Nishiyama

Abstract read
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In one paragraph

Article in Extremophiles : life under extreme conditions, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Kento TakahashiBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Fumika NakanishiBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Takeo TomitaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Nagisa AkiyamaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Kerstin LassakMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Sonja-Verena AlbersMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Tomohisa KuzuyamaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Makoto NishiyamaBiotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. umanis@mail.ecc.u-tokyo.ac.jp.
The University of Tokyo · JPMax Planck Institute for Terrestrial Microbiology · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sulfolobus acidocaldarius, a hyperthermoacidophilic archaeon, possesses two β-decarboxylating dehydrogenase genes, saci_0600 and saci_2375, in its genome, which suggests that it uses these enzymes for three similar reactions in lysine biosynthesis through 2-aminoadipate, leucine biosynthesis, and the tricarboxylic acid cycle. To elucidate their roles, these two genes were expressed in Escherichia coli in the present study and their gene products were characterized. Saci_0600 recognized 3-isopropylmalate as a substrate, but exhibited slight and no activity for homoisocitrate and isocitrate, respectively. Saci_2375 exhibited distinct and similar activities for isocitrate and homoisocitrate, but no detectable activity for 3-isopropylmalate. These results suggest that Saci_0600 is a 3-isopropylmalate dehydrogenase for leucine biosynthesis and Saci_2375 is a dual function enzyme serving as isocitrate-homoisocitrate dehydrogenase. The crystal structure of Saci_0600 was determined as a closed-form complex that binds 3-isopropylmalate and Mg

Indexed as

3-Isopropylmalate DehydrogenaseBacterial ProteinsIsocitrate DehydrogenaseIsocitratesMagnesiumMalatesProtein BindingSulfolobus acidocaldarius3-Isopropylmalate DehydrogenaseBacterial Proteinsbeta-isopropylmalateIsocitrate DehydrogenaseIsocitratesisocitric acidMagnesiumMalates3-Isopropylmalate dehydrogenaseCrystal structureHomoisocitrate dehydrogenaseIsocitrate dehydrogenaseSulfolobus acidocaldariusβ-Decarboxylating dehydrogenase

Identifiers

PMID27590116
OpenAlexW2512120218

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.