ArticlePLoS genetics2016
DNA Replication Control Is Linked to Genomic Positioning of Control Regions in Escherichia coli.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- The Escherichia coli replication initiator DnaA is titrated on the chromosome.Nature communications · 2025Article
- Dispensability of extrinsic DnaA regulators inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Direct single-cell observation of a keyScience advances · 2024Article
- The replication enhancer crtS depends on transcription factor Lrp for modulating binding of initiator RctB to ori2 of Vibrio cholerae.Nucleic acids research · 2024Article
- Cell cycle-coordinated maintenance of theEcoSal Plus · 2023Review
- Open Questions about the Roles of DnaA, Related Proteins, and Hyperstructure Dynamics in the Cell Cycle.Life (Basel, Switzerland) · 2023Review
- Regulatory elements coordinating initiation of chromosome replication to theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Robust replication initiation from coupled homeostatic mechanisms.Nature communications · 2022Article
- The dimerization interface of initiator RctB governs chaperone and enhancer dependence of Vibrio cholerae chromosome 2 replication.Nucleic acids research · 2022Article
- Negative feedback for DARS2-Fis complex by ATP-DnaA supports the cell cycle-coordinated regulation for chromosome replication.Nucleic acids research · 2021Article
- Arresting chromosome replication upon energy starvation in Escherichia coli.Current genetics · 2021Review
- New Insights into the Antimicrobial Action of Cinnamaldehyde towardsBiomolecules · 2021Article
- Blocking, Bending, and Binding: Regulation of Initiation of Chromosome Replication During theFrontiers in microbiology · 2021Review
- Cell Size Is Coordinated with Cell Cycle by Regulating Initiator Protein DnaA in E. coli.Biophysical journal · 2020Article
- A novel mode of DnaA-DnaA interaction promotes ADP dissociation for reactivation of replication initiation activity.Nucleic acids research · 2019Article
- Countermeasures to survive excessive chromosome replication in Escherichia coli.Current genetics · 2018Review
- Impact of Chromosomal Architecture on the Function and Evolution of Bacterial Genomes.Frontiers in microbiology · 2018Review
- The DnaA Tale.Frontiers in microbiology · 2018Review
- Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach.Journal of visualized experiments : JoVE · 2017Article
- Control of bacterial chromosome replication by non-coding regions outside the origin.Current genetics · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome replication in Escherichia coli is in part controlled by three non-coding genomic sequences, DARS1, DARS2, and datA that modulate the activity of the initiator protein DnaA. The relative distance from oriC to the non-coding regions are conserved among E. coli species, despite large variations in genome size. Here we use a combination of i) site directed translocation of each region to new positions on the bacterial chromosome and ii) random transposon mediated translocation followed by culture evolution, to show genetic evidence for the importance of position. Here we provide evidence that the genomic locations of these regulatory sequences are important for cell cycle control and bacterial fitness. In addition, our work shows that the functionally redundant DARS1 and DARS2 regions play different roles in replication control. DARS1 is mainly involved in maintaining the origin concentration, whether DARS2 is also involved in maintaining single cell synchrony.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.