ArticleJournal of experimental botany2016
Plastidic phosphoglucomutase and ADP-glucose pyrophosphorylase mutants impair starch synthesis in rice pollen grains and cause male sterility.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
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Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 122 citations in OpenAlex.
- Pooled it
- The OsWRKY15-OsSUT3 Regulatory Module Enhances Rice Pollen Fertility by Mediating Sucrose Allocation to Anthers.Plant biotechnology journal · 2026Article
- HSP101-encoding NEO-TETRAPLOID RICE FERTILITY GENE 1 regulates tapetum development through interaction with SAPK2 in polyploid rice.Journal of integrative plant biology · 2026Article
- Histone variant H2A.X represses deposition of active H3K4me3 marks and restricts H2A.W incorporation.Nucleic acids research · 2026Article
- Moderate Regulation of SBEIIb Gene Expression by Editing Cis-Regulatory Elements Enhances the Resistant Starch Content in Rice.Plant biotechnology journal · 2026Article
- A plastidic starch biosynthetic pathway defines columella-specific carbon metabolism in rice root tips.Frontiers in plant science · 2026Article
- Ectopic expression of maize gene ZmDUF1645 in rice affects rice grain traits and cold tolerance.BMC plant biology · 2025Article
- CRISPR-driven innovations in rice (Oryza sativa L.) breeding: precision development of male sterile lines.Molecular biology reports · 2025Review
- Article
- Genetic and molecular mechanisms underlying the male sterility in rice.Journal of applied genetics · 2025Review
- Anther Transcriptome Analysis of Two Heat Tolerance-Differentiated Indica Rice Restorer Lines Reveals the Importance of Non-Structural Carbohydrates and ATP in the Regulation of Heat Tolerance.International journal of molecular sciences · 2025Article
- Molecular Mechanisms of Grain Chalkiness Variation in Rice Panicles.Plants (Basel, Switzerland) · 2025Article
- The rice microRNA159-SPOROCYTELESS EAR2 module regulates starch biosynthesis during pollen development and maintains male fertility.The Plant cell · 2024Article
- LAZY4 acts additively with the starch-statolith-dependent gravity-sensing pathway to regulate shoot gravitropism and tiller angle in rice.Plant communications · 2024Article
- Comparative Analysis of Hulless Barley Transcriptomes to Regulatory Effects of Phosphorous Deficiency.Life (Basel, Switzerland) · 2024Article
- Chalkiness and premature controlled by energy homeostasis in OsNAC02 Ko-mutant during vegetative endosperm development.BMC plant biology · 2024Article
- BOTRYOID POLLEN 1 regulates ROS-triggered PCD and pollen wall development by controlling UDP-sugar homeostasis in rice.The Plant cell · 2023Article
- Homeostasis of flavonoids and triterpenoids most likely modulates starch metabolism for pollen tube penetration in rice.Plant biotechnology journal · 2023Article
- Rice HEAT SHOCK PROTEIN60-3B maintains male fertility under high temperature by starch granule biogenesis.Plant physiology · 2023Article
- A starch- and ROS-regulating heat shock protein helps maintain male fertility in heat-stressed rice plants.Plant physiology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To elucidate the starch synthesis pathway and the role of this reserve in rice pollen, we characterized mutations in the plastidic phosphoglucomutase, OspPGM, and the plastidic large subunit of ADP-glucose (ADP-Glc) pyrophosphorylase, OsAGPL4 Both genes were up-regulated in maturing pollen, a stage when starch begins to accumulate. Progeny analysis of self-pollinated heterozygous lines carrying the OspPGM mutant alleles, osppgm-1 and osppgm-2, or the OsAGPL4 mutant allele, osagpl4-1, as well as reciprocal crosses between the wild type (WT) and heterozygotes revealed that loss of OspPGM or OsAGPL4 caused male sterility, with the former condition rescued by the introduction of the WT OspPGM gene. While iodine staining and transmission electron microscopy analyses of pollen grains from homozygous osppgm-1 lines produced by anther culture confirmed the starch null phenotype, pollen from homozygous osagpl4 mutant lines, osagpl4-2 and osagpl4-3, generated by the CRISPR/Cas system, accumulated small amounts of starch which were sufficient to produce viable seed. Such osagpl4 mutant pollen, however, was unable to compete against WT pollen successfully, validating the important role of this reserve in fertilization. Our results demonstrate that starch is mainly polymerized from ADP-Glc synthesized from plastidic hexose phosphates in rice pollen and that starch is an essential requirement for successful fertilization in rice.
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