ArticleZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi2016
[Effect of miR- 155 on immune- factors and its mechanism in mesenchymal stem cells under hypoxic environment].
Article in Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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8 authors at 1 institution in 1 country.
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Abstract
objectiveTo investigate the effect of miR-155 on immune-factors and its mechanism in mesenchymal stem cells under hypoxia.
methodsThe microRNA sequences targeting miR-155 mimic and mimic NC gene was designed and transfected into MSC by lipofectamineTM 2000. Lipopolysaccharide was used to stimulate the immunity of MSC under hypoxic environment. Transfection efficiency of miR- 155 and immune-related genes (IL-6, IL-8, iNOS, TGF-β, HIF-1α) were detected by real-time RT-PCR. The cell surface antigens (CD29, CD73, CD90, CD105, CD31, CD45) and supernatant cytokines (IL- 6, IL- 8, TGF- β, SDF- 1α) were analyzed by flow cytometry and ELISA, respectively. Western Blot was applied to evaluate related proteins, iNOS and HIF- 1α.
resultmiR- 155 was transfected into MSC effectively (53.447±8.361 vs 1.070±0.174, P<0.01). In miR-155 high-expressed groups, the expressions of IL-6 and IL-8 were up-regulated [(24.201±1.536) vs (1.802±0.058), P<0.01; (24.406±4.611) vs (7.407± 1.553), P<0.01] and iNOS was markedly suppressed [(0.151 ±0.035) vs (32.925±1.632), P<0.01]. Hypoxia up-regulated expressions of HIF-1α [(45.093±3.371) vs (2.210±0.498), P<0.01] and promoted the regulation of miR-155. MiR-155 and hypoxia had effect on mRNA expression of SDF-1α and TGF-β [(5.690±1.655) vs (0.841±0.194), P<0.01; (6.982±1.353) vs (0.632±0.184), P<0.01]. However, there was no influence on cytokines of SDF- 1α and TGF- β [(24.609±2.584) vs (25.359±2.455), P=0.760;(0.568±0.019) vs (0.345±0.037), P=0.002].
conclusionHypoxia environment may promote miR-155 to positively modulate immune factors of MSC through up-regulating the expression of HIF-1α, which down-regulated iNOS protein.
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