ArticleJournal of assisted reproduction and genetics2016
Freeze/thaw stress induces organelle remodeling and membrane recycling in cryopreserved human mature oocytes.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Research Progress on Mammalian Oocyte Vitrification: From Damage Mechanisms to Optimization Strategies.Animals : an open access journal from MDPI · 2026Review
- Dual-omics reveals temporal translational recovery landscapes and cryodamage repair mechanisms in vitrified mouse oocytes.Journal of assisted reproduction and genetics · 2025Article
- Multi-omics analysis reveals a protective role of endogenous proline in sheep oocyte vitrification and its therapeutic application.Frontiers in cell and developmental biology · 2025Article
- Mitochondrial transplantation: a promising strategy for treating degenerative joint diseases.Journal of translational medicine · 2024Review
- Article
- Effect of ovarian growth factors on ultra-structural maturation in frozen human immature oocytes after in vitro maturation: a comparative study.Reproductive health · 2022Article
- Cryopreservation of Gametes and Embryos and Their Molecular Changes.International journal of molecular sciences · 2021Review
- Vitrification of camel oocytes transiently impacts mitochondrial functions without affecting the developmental potential after intracytoplasmic sperm injection and parthenogenetic activation.Environmental science and pollution research international · 2020Article
- Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures.International journal of environmental research and public health · 2020Article
- Stepped vitrification technique for human ovarian tissue cryopreservation.Scientific reports · 2019Article
- Oxygen concentration alters mitochondrial structure and function in in vitro fertilized preimplantation mouse embryos.Human reproduction (Oxford, England) · 2019Article
- Cryobiology in human ARTs: then and now.Journal of assisted reproduction and genetics · 2018Article
- The importance of post-thaw subculture for standardizing cellular activity of fresh or cryopreserved mouse embryonic stem cells.Asian-Australasian journal of animal sciences · 2018Article
- Mancozeb impairs the ultrastructure of mouse granulosa cells in a dose-dependent manner.The Journal of reproduction and development · 2018Article
- Vitrification of human immature oocytes before and after in vitro maturation: a review.Journal of assisted reproduction and genetics · 2017Review
- Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation.The Journal of reproduction and development · 2017Article
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Authors and funding
9 authors.
Funding
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Abstract
purposeOur aim was to evaluate the ultrastructure of human metaphase II oocytes subjected to slow freezing and fixed after thawing at different intervals during post-thaw rehydration.
methodsSamples were studied by light and transmission electron microscopy.
resultsWe found that vacuolization was present in all cryopreserved oocytes, reaching a maximum in the intermediate stage of rehydration. Mitochondria-smooth endoplasmic reticulum (M-SER) aggregates decreased following thawing, particularly in the first and intermediate stages of rehydration, whereas mitochondria-vesicle (MV) complexes augmented in the same stages. At the end of rehydration, vacuoles and MV complexes both diminished and M-SER aggregates increased again. Cortical granules (CGs) were scarce in all cryopreserved oocytes, gradually diminishing as rehydration progressed.
conclusionsThis study also shows that such a membrane remodeling is mainly represented by a dynamic process of transition between M-SER aggregates and MV complexes, both able of transforming into each other. Vacuoles and CG membranes may take part in the membrane recycling mechanism.
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