ArticleMethods in enzymology2016
Metal-Directed Design of Supramolecular Protein Assemblies.
Article in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Programmable DNA Assemblies Reconstitute Supramolecular Protein Function.bioRxiv : the preprint server for biology · 2026Article
- Pulse Dipolar Electron Paramagnetic Resonance Spectroscopy Reveals Buffer-Modulated Cooperativity of Metal-Templated Protein Dimerization.The journal of physical chemistry letters · 2022Article
- Supramolecular assembly of protein building blocks: from folding to function.Nano convergence · 2022Review
- Protein Assembly by Design.Chemical reviews · 2021Review
- Experimental and theoretical study on converting myoglobin into a stable domain-swapped dimer by utilizing a tight hydrogen bond network at the hinge region.RSC advances · 2021Article
- Biofabrication of functional protein nanoparticles through simple His-tag engineering.ACS sustainable chemistry & engineering · 2021Article
- Design of metal-mediated protein assemblies via hydroxamic acid functionalities.Nature protocols · 2021Article
- Article
- Heteromeric three-stranded coiled coils designed using a Pb(II)(Cys)Nature chemistry · 2020Article
- Bottom-up synthesis of protein-based nanomaterials from engineered β-solenoid proteins.PloS one · 2020Article
- Better together: building protein oligomers naturally and by design.Biochemical Society transactions · 2019Review
- Employing 25-Residue Docking Motifs from Modular Polyketide Synthases as Orthogonal Protein Connectors.ACS synthetic biology · 2019Article
- Metal-dependent assembly of a protein nano-cage.Protein science : a publication of the Protein Society · 2019Article
- Determining the Structural and Energetic Basis of Allostery in a De Novo Designed Metalloprotein Assembly.Journal of the American Chemical Society · 2018Article
- Engineering the entropy-driven free-energy landscape of a dynamic nanoporous protein assembly.Nature chemistry · 2018Article
- Hierarchical design of artificial proteins and complexes toward synthetic structural biology.Biophysical reviews · 2018Review
- Control of Amphiphile Self-Assembly via Bioinspired Metal Ion Coordination.Journal of the American Chemical Society · 2018Article
- De Novo Design of an Allosteric Metalloprotein Assembly with Strained Disulfide Bonds.Journal of the American Chemical Society · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Owing to their central roles in cellular signaling, construction, and biochemistry, protein-protein interactions (PPIs) and protein self-assembly have become a major focus of molecular design and synthetic biology. In order to circumvent the complexity of constructing extensive noncovalent interfaces, which are typically involved in natural PPIs and protein self-assembly, we have developed two design strategies, metal-directed protein self-assembly (MDPSA) and metal-templated interface redesign (MeTIR). These strategies, inspired by both the proposed evolutionary roles of metals and their prevalence in natural PPIs, take advantage of the favorable properties of metal coordination (bonding strength, directionality, and reversibility) to guide protein self-assembly with minimal design and engineering. Using a small, monomeric protein (cytochrome cb562) as a model building block, we employed MDPSA and MeTIR to create a diverse array of functional supramolecular architectures which range from structurally tunable oligomers to metalloprotein complexes that can properly self-assemble in living cells into novel metalloenzymes. The design principles and strategies outlined herein should be readily applicable to other protein systems with the goal of creating new PPIs and protein assemblies with structures and functions not yet produced by natural evolution.
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