ArticleMethods in enzymology2016
Engineering and Application of LOV2-Based Photoswitches.
Article in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Emerging roles of transcriptional condensates as temporal signal integrators.Nature reviews. Genetics · 2025Review
- Spatiotemporal Optical Control of Gαq-PLCβ Interactions.ACS synthetic biology · 2024Article
- Article
- Allosteric regulation of kinase activity in living cells.bioRxiv : the preprint server for biology · 2023Article
- EVL and MIM/MTSS1 regulate actin cytoskeletal remodeling to promote dendritic filopodia in neurons.The Journal of cell biology · 2023Article
- A rationally designed optochemogenetic switch for activating canonical Wnt signaling.iScience · 2023Article
- LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics.Biophysics and physicobiology · 2023Article
- Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues.Nature communications · 2022Article
- Transcytosis and trans-synaptic retention by postsynaptic ErbB4 underlie axonal accumulation of NRG3.The Journal of cell biology · 2022Article
- Optophysiology: Illuminating cell physiology with optogenetics.Physiological reviews · 2022Review
- The Next Frontier for Designing Switchable Proteins: Rational Enhancement of Kinetics.The journal of physical chemistry. B · 2021Article
- Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein.eLife · 2020Article
- Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling.Nature communications · 2020Article
- Engineering Optogenetic Protein Analogs.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution.ACS synthetic biology · 2018Article
- Light-Dependent Cytoplasmic Recruitment Enhances the Dynamic Range of a Nuclear Import Photoswitch.Chembiochem : a European journal of chemical biology · 2018Article
- Cells lay their own tracks - optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration.Journal of cell science · 2017Article
- An engineered photoswitchable mammalian pyruvate kinase.The FEBS journal · 2017Article
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Authors and funding
3 authors.
Funding
Abstract
Cellular optogenetic switches, a novel class of biological tools, have improved our understanding of biological phenomena that were previously intractable. While the design and engineering of these proteins has historically varied, they are all based on borrowed elements from plant and bacterial photoreceptors. In general terms, each of the optogenetic switches designed to date exploits the endogenous light-induced change in photoreceptor conformation while repurposing its effect to target a different biological phenomenon. We focus on the well-characterized light-oxygen-voltage 2 (LOV2) domain from Avena sativa phototropin 1 as our cornerstone for design. While the function of the LOV2 domain in the context of the phototropin protein is not fully elucidated, its thorough biophysical characterization as an isolated domain has created a strong foundation for engineering of photoswitches. In this chapter, we examine the biophysical characteristics of the LOV2 domain that may be exploited to produce an optogenetic switch and summarize previous design efforts to provide guidelines for an effective design. Furthermore, we provide protocols for assays including fluorescence polarization, phage display, and microscopy that are optimized for validating, improving, and using newly designed photoswitches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.