ArticleMicrobial cell factories2016
A β-glucosidase hyper-production Trichoderma reesei mutant reveals a potential role of cel3D in cellulase production.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 108 citations in OpenAlex.
- Heterologous Expression of aJournal of fungi (Basel, Switzerland) · 2026Article
- Bioaugmentation strategies for methane production from digestate-alkali pretreated biomass via anaerobic digestion.Bioresources and bioprocessing · 2025Article
- Investigating the cellular functions of β-Glucosidases for synthesis of lignocellulose-degrading enzymes inEngineering microbiology · 2023Article
- Profiling of the β-glucosidases identified in the genome ofApplied and environmental microbiology · 2023Article
- Intracellular Sugar Transporters Facilitate Cellulase Synthesis inBiomolecules · 2023Article
- Discovery of ER-localized sugar transporters for cellulase production with lac1 being essential.Biotechnology for biofuels and bioproducts · 2022Article
- Induction of cellulase production by SrBioresources and bioprocessing · 2022Article
- Sorbicillinoids hyperproduction without affecting the cellulosic enzyme production in Trichoderma reesei JNTR5.Biotechnology for biofuels and bioproducts · 2022Article
- Improvement in the Thermostability of a Recombinant β-Glucosidase Immobilized in Zeolite under Different Conditions.Molecules (Basel, Switzerland) · 2022Article
- Transmembrane transport process and endoplasmic reticulum function facilitate the role of gene cel1b in cellulase production of Trichoderma reesei.Microbial cell factories · 2022Article
- Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi.Biotechnology for biofuels and bioproducts · 2022Article
- Mechanism of differential expression of β-glucosidase genes in functional microbial communities in response to carbon catabolite repression.Biotechnology for biofuels and bioproducts · 2022Article
- Development of a powerful synthetic hybrid promoter to improve the cellulase system of Trichoderma reesei for efficient saccharification of corncob residues.Microbial cell factories · 2022Article
- From induction to secretion: a complicated route for cellulase production in Trichoderma reesei.Bioresources and bioprocessing · 2021Review
- Glutamine involvement in nitrogen regulation of cellulase production in fungi.Biotechnology for biofuels · 2021Article
- Article
- Rational engineering of xylanase hyper-producing system in Trichoderma reesei for efficient biomass degradation.Biotechnology for biofuels · 2021Article
- High-dose rapamycin exerts a temporary impact on T. reesei RUT-C30 through gene trFKBP12.Biotechnology for biofuels · 2021Article
- β-Glucosidase genes differentially expressed during composting.Biotechnology for biofuels · 2020Article
- Involvement ofFrontiers in microbiology · 2020Article
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe conversion of cellulose by cellulase to fermentable sugars for biomass-based products such as cellulosic biofuels, biobased fine chemicals and medicines is an environment-friendly and sustainable process, making wastes profitable and bringing economic benefits. Trichoderma reesei is the well-known major workhorse for cellulase production in industry, but the low β-glucosidase activity in T. reesei cellulase leads to inefficiency in biomass degradation and limits its industrial application. Thus, there are ongoing interests in research to develop methods to overcome this insufficiency. Moreover, although β-glucosidases have been demonstrated to influence cellulase production and participate in the regulation of cellulase production, the underlying mechanism remains unclear.
resultsThe T. reesei recombinant strain TRB1 was constructed from T. reesei RUT-C30 by the T-DNA-based mutagenesis. Compared to RUT-C30, TRB1 displays a significant enhancement of extracellular β-glucosidase (BGL1) activity with 17-fold increase, a moderate increase of both the endoglucanase (EG) activity and the exoglucanase (CBH) activity, a minor improvement of the total filter paper activity, and a faster cellulase induction. This superiority of TRB1 over RUT-C30 is independent on carbon sources and improves the saccharification ability of TRB1 cellulase on pretreated corn stover. Furthermore, TRB1 shows better resistance to carbon catabolite repression than RUT-C30. Secretome characterization of TRB1 shows that the amount of CBH, EG and BGL in the supernatant of T. reesei TRB1 was indeed increased along with the enhanced activities of these three enzymes. Surprisingly, qRT-PCR and gene cloning showed that in TRB1 β-glucosidase cel3D was mutated through the random insertion by AMT and was not expressed.
conclusionsThe T. reesei recombinant strain TRB1 constructed in this study is more desirable for industrial application than the parental strain RUT-C30, showing extracellular β-glucosidase hyper production, high cellulase production within a shorter time and a better resistance to carbon catabolite repression. Disruption of β-glucosidase cel3D in TRB1 was identified, which might contribute to the superiority of TRB1 over RUT-C30 and might play a role in the cellulase production. These results laid a foundation for future investigations to further improve cellulase enzymatic efficiency and reduce cost for T. reesei cellulase production.
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