ArticleJBRA assisted reproduction2016
A new micro swim-up procedure for sperm preparation in ICSI treatments: preliminary microbiological testing.
Article in JBRA assisted reproduction, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- A fast and safe technique for sperm preparation in ICSI treatments within a randomized controlled trial (RCT).Reproductive biology and endocrinology : RB&E · 2020Trial
- Next-Generation Sequencing to Elucidate the Semen Microbiome in Male Reproductive Disorders.Medicina (Kaunas, Lithuania) · 2023Review
- The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure.Reproductive biology and endocrinology : RB&E · 2020Article
- Microbial contamination in assisted reproductive technology: source, prevalence, and cost.Journal of assisted reproduction and genetics · 2020Review
- The seminal microbiome in health and disease.Nature reviews. Urology · 2019Review
- Comparison of in vitro fertilization outcomes in ICSI cycles after human sperm preparation by density gradient centrifugation and direct micro swim-up without centrifugation.JBRA assisted reproduction · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study aimed to assess the levels of microbial contamination in semen samples before and after the micro swim-up (MSU) procedure in intra-cytoplasmic sperm injection (ICSI). The new method is an upgrade to the classic wash swim-up procedure.
methodsSemen analysis and microbiological tests were carried out before and after the MSU procedure. A total of twenty semen samples were analyzed.
resultsPathogens were observed in semen samples only before MSU and never after ICSI. Microbiological tests revealed a large prevalence of gram-positive cocci [Staphylococcus spp. (n=16, 80%) and viridans streptococci (n=10, 50%)]. The results of this study indicate that direct MSU in ICSI improved the ICSI workflow.
conclusionThe new workflow is faster and more affordable, and is likely to prevent infection problems that could arise from the normal microbial flora of the semen.
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Registered trials
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