ArticleNPJ breast cancer
A microchip platform for structural oncology applications.
Article in NPJ breast cancer. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials.Chembiochem : a European journal of chemical biology · 2022Article
- Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy.Nanoscale · 2021Article
- Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- RETRACTED: Structural analysis of BRCA1 reveals modification hotspot.Science advances · 2017Article
- Tunable substrates improve imaging of viruses and cancer proteins.Microscopy today · 2017Article
- Molecular Analysis of BRCA1 in Human Breast Cancer Cells Under Oxidative Stress.Scientific reports · 2017Article
- Preparation of Disease-Related Protein Assemblies for Single Particle Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Recent advances in the development of functional materials offer new tools to dissect human health and disease mechanisms. The use of tunable surfaces is especially appealing as substrates can be tailored to fit applications involving specific cell types or tissues. Here we use tunable materials to facilitate the three-dimensional (3D) analysis of BRCA1 gene regulatory complexes derived from human cancer cells. We employed a recently developed microchip platform to isolate BRCA1 protein assemblies natively formed in breast cancer cells with and without BRCA1 mutations. The captured assemblies proved amenable to cryo-electron microscopy (EM) imaging and downstream computational analysis. Resulting 3D structures reveal the manner in which wild-type BRCA1 engages the RNA polymerase II (RNAP II) core complex that contained K63-linked ubiquitin moieties-a putative signal for DNA repair. Importantly, we also determined that molecular assemblies harboring the
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.