ArticleBiotechnology for biofuels2016
The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 93 citations in OpenAlex.
- Structural proteomics reveals the functional docking interface of ferredoxin-NADPThe Plant journal : for cell and molecular biology · 2026Article
- Smart-driven bioengineering techniques for enhancing microalgal biohydrogen production.Biotechnology for biofuels and bioproducts · 2026Review
- Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.Applied microbiology and biotechnology · 2026Article
- Outlook on Synthetic Biology-Driven Hydrogen Production: Lessons from Algal Photosynthesis Applied to Cyanobacteria.Energy & fuels : an American Chemical Society journal · 2025Review
- Light-Driven HPlants (Basel, Switzerland) · 2024Review
- Review
- Novel concepts and engineering strategies for heterologous expression of efficient hydrogenases in photosynthetic microorganisms.Frontiers in microbiology · 2023Review
- A two-phase protocol for ambient hydrogen production usingSTAR protocols · 2022Article
- Recent advances in utilization of ferredoxins for biosynthesis of valuable compounds.World journal of microbiology & biotechnology · 2022Review
- Synthetic biology for improved hydrogen production in Chlamydomonas reinhardtii.Microbial biotechnology · 2022Review
- Photosystem I light-harvesting proteins regulate photosynthetic electron transfer and hydrogen production.Plant physiology · 2022Article
- Investigation of the Ferredoxin's Influence on the Anaerobic and Aerobic, Enzymatic HFrontiers in bioengineering and biotechnology · 2021Article
- Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression inFrontiers in plant science · 2020Article
- Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research.Frontiers in plant science · 2020Article
- Metalloprotein switches that display chemical-dependent electron transfer in cells.Nature chemical biology · 2019Article
- Article
- EngineeredBiotechnology for biofuels · 2019Article
- Re-routing photosynthetic energy for continuous hydrogen production in vivo.Biotechnology for biofuels · 2019Article
- The Integration of Multiple Nuclear-Encoded Transgenes in the Green AlgaFrontiers in plant science · 2019Article
- Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen.Photosynthesis research · 2018Article
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Authors and funding
9 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHydrogen photo-production in green algae, catalyzed by the enzyme [FeFe]-hydrogenase (HydA), is considered a promising source of renewable clean energy. Yet, a significant increase in hydrogen production efficiency is necessary for industrial scale-up. We have previously shown that a major challenge to be resolved is the inferior competitiveness of HydA with NADPH production, catalyzed by ferredoxin-NADP(+)-reductase (FNR). In this work, we explored the in vivo hydrogen production efficiency of Fd-HydA, where the electron donor ferredoxin (Fd) is fused to HydA and expressed in the model organism Chlamydomonas reinhardtii.
resultsWe show that once the Fd-HydA fusion gene is expressed in micro-algal cells of C. reinhardtii, the fusion enzyme is able to intercept photosynthetic electrons and use them for efficient hydrogen production, thus supporting the previous observations made in vitro. We found that Fd-HydA has a ~4.5-fold greater photosynthetic hydrogen production rate standardized for hydrogenase amount (PHPRH) than that of the native HydA in vivo. Furthermore, we provide evidence suggesting that the fusion protein is more resistant to oxygen than the native HydA.
conclusionsThe in vivo photosynthetic activity of the Fd-HydA enzyme surpasses that of the native HydA and shows higher oxygen tolerance. Therefore, our results provide a solid platform for further engineering efforts towards efficient hydrogen production in microalgae through the expression of synthetic enzymes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.