ArticleJournal of preventive medicine and hygiene2016
PCR-based assay for the rapid and precise distinction of Pseudomonas aeruginosa from other Pseudomonas species recovered from burns patients.
Article in Journal of preventive medicine and hygiene, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Molecular and computational analysis of conserved outer membranes (oprI and oprL) in MDR clinical isolates of Pseudomonas aeruginosa as potential vaccine targets from Lahore, Pakistan.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Role of MexAB-OprM and MexXY-OprM efflux pumps in carbapenem-resistant Pseudomonas aeruginosa from burn wounds.Molecular biology reports · 2026Article
- Molecular and genomic insights into multidrug-resistant (MDR) and extensively drug-resistant (XDR) Pseudomonas aeruginosa causing burn wound infections in Bangladesh.Scientific reports · 2025Article
- Survival strategies of imipenem-resistant P. aeruginosa: a comparative analysis of surface-bound biofilms and unbound aggregates.The Journal of antibiotics · 2025Article
- Draft genome sequence ofMicrobiology resource announcements · 2025Article
- Miniaturized Devices for Isothermal Amplification and Photometric Quantification of Pseudomonas Aeruginosa.IEEE open journal of engineering in medicine and biology · 2025Article
- Antimicrobial resistance, virulence gene profiling, and genetic diversity of multidrug-resistant Pseudomonas aeruginosa isolates in Mazandaran, Iran.BMC microbiology · 2024Article
- Article
- The impact of quorum sensing and biofilm formation on antimicrobial resistance and virulence of XDR and MDRIranian journal of veterinary research · 2024Article
- Isolation of Lead Resistant Bacteria from Spent Mushroom Compost and Their Impact on Growth and Biochemical Parameters of Safflower.Current microbiology · 2023Article
- Detection of Antibiotic Resistance Genes inInfection and drug resistance · 2022Article
- Characterization of the virulence of Pseudomonas aeruginosa strains causing ventilator-associated pneumonia.BMC infectious diseases · 2020Article
- Virulence Constitution of Multi-Drug-ResistantInfection and drug resistance · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPseudomonas aeruginosa is an important lifethreatening nosocomial pathogen which plays a prominent role in wound infections in burns patients. We designed this study to identify the isolates of P. aeruginosa recovered from burns patients at the genus and species levels by means of primers targeting oprI and oprL genes.
methodsDuring a 5-month period, wound samples were taken from burns patients and plated on MacConkey agar. All suspected colonies were screened for P. aeruginosa by means of a combination of phenotype tests. Specific primers for oprI and oprL genes were then used for the molecular identification of colonies.
resultsDuring the 5-month period, bacterial isolates recovered from burn wound infections were analyzed. Phenotype identification tests identified 171 (34.8%) P. aeruginosa isolates. However, molecular techniques that used species-specific primers to detect the amplicon of the oprL gene confirmed the exact identification of P. aeruginosa in only 133 cases; in the other isolates, the use of genus-specific primers detected the amplicon of the oprI gene, which confirmed the identification of fluorescent pseudomonads.
conclusionsThis study indicates that molecular detection by means of an assay targeting the oprL gene is a useful technique for the rapid and precise detection of P. aeruginosa in burns patients. In addition to phenotype testing, PCR detection should be carried out in order to promptly ascertain the best aggressive antibiotic therapy for P. aeruginosa infections, thereby significantly improving clinical outcomes.
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Identifiers
27582633PMC4996044W2509139052What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.