ArticleJournal of clinical microbiology2016
Identification and Analysis of Informative Single Nucleotide Polymorphisms in 16S rRNA Gene Sequences of the Bacillus cereus Group.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- The Human Microbiota and Obesity: A Literature Systematic Review of In Vivo Models and Technical Approaches.International journal of molecular sciences · 2018Pooled it
- EndoNUclease Heteroduplex cleavage typing a new technique for rapid typing of bacterial isolates in the context of nosocomial outbreaks: proof of concept withFrontiers in cellular and infection microbiology · 2026Article
- Superior Resolution Profiling of the Coleofasciculus Microbiome by Amplicon Sequencing of the Complete 16S rRNA Gene and ITS Region.Environmental microbiology reports · 2025Article
- Utilizing novel Escherichia coli-specific conserved signature proteins for enhanced monitoring of recreational water quality.MicrobiologyOpen · 2024Article
- Exploring the Feasibility of 16S rRNA Short Amplicon Sequencing-Based Microbiota Analysis for Microbiological Safety Assessment of Raw Oyster.Journal of microbiology and biotechnology · 2023Article
- Molecular Identification ofAnimals : an open access journal from MDPI · 2022Article
- Whole-Genome and Transcriptome Sequencing-Based Characterization ofFrontiers in microbiology · 2022Article
- Ultrasensitive Detection ofInternational journal of molecular sciences · 2021Article
- The diversity and distribution of D1 proteins in cyanobacteria.Photosynthesis research · 2020Article
- Molecular characterization of B. anthracis isolates from the anthrax outbreak among cattle in Karnataka, India.BMC microbiology · 2020Article
- Characterization ofToxins · 2020Review
- Single Core Genome Sequencing for Detection of bothInternational journal of environmental research and public health · 2019Article
- The ftsA gene as a molecular marker for phylogenetic studies in Bradyrhizobium and identification of Bradyrhizobium japonicum.Journal of applied genetics · 2019Article
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Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of 16S rRNA genes is important for phylogenetic classification of known and novel bacterial genera and species and for detection of uncultivable bacteria. PCR amplification of 16S rRNA genes with universal primers produces a mixture of amplicons from all rRNA operons in the genome, and the sequence data generally yield a consensus sequence. Here we describe valuable data that are missing from consensus sequences, variable effects on sequence data generated from nonidentical 16S rRNA amplicons, and the appearance of data displayed by different software programs. These effects are illustrated by analysis of 16S rRNA genes from 50 strains of the Bacillus cereus group, i.e., Bacillus anthracis, Bacillus cereus, Bacillus mycoides, and Bacillus thuringiensis These species have 11 to 14 rRNA operons, and sequence variability occurs among the multiple 16S rRNA genes. A single nucleotide polymorphism (SNP) previously reported to be specific to B. anthracis was detected in some B. cereus strains. However, a different SNP, at position 1139, was identified as being specific to B. anthracis, which is a biothreat agent with high mortality rates. Compared with visual analysis of the electropherograms, basecaller software frequently missed gene sequence variations or could not identify variant bases due to overlapping basecalls. Accurate detection of 16S rRNA gene sequences that include intragenomic variations can improve discrimination among closely related species, improve the utility of 16S rRNA databases, and facilitate rapid bacterial identification by targeted DNA sequence analysis or by whole-genome sequencing performed by clinical or reference laboratories.
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Registered trials
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