Evidence map›Paper›PMID 27581683›Full record

ArticleCellular and molecular neurobiology2017

Effects of Alpha-Synuclein on Primary Spinal Cord Neurons Associated with Apoptosis and CNTF Expression.

Guo-Ying Feng, Jia Liu, You-Cui Wang, Zhen-Yu Wang, Yue Hu, Qing-Jie Xia, Yang Xu, Fei-Fei Shang, Mei-Rong Chen, Fang Wang and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular neurobiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.5field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
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  6. Neural regeneration research · 2017
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Guo-Ying FengDepartment of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China.
Jia LiuInstitute of Neuroscience, Kunming Medical University, Kunming, 650031, China.
You-Cui WangDepartment of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Zhen-Yu WangBiomedical Engineering Research Center, Kunming Medical University, Kunming, 650031, China.
Yue HuDepartment of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Qing-Jie XiaDepartment of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Yang XuDepartment of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Fei-Fei ShangDepartment of Anesthesia, Institute of Neurological Disease, Translational Neuroscience Center, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Mei-Rong ChenInstitute of Neuroscience, Kunming Medical University, Kunming, 650031, China.
Fang WangInstitute of Neuroscience, Kunming Medical University, Kunming, 650031, China. 56885606@qq.com.
Xue ZhouDepartment of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. zhouxue7239@163.com.
Ting-Hua WangDepartment of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. 371854427@qq.com.
Sichuan University · CNKunming Medical University · CNWest China Hospital of Sichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spinal cord injury (SCI) often causes neurological deficits with poor recovery; the treatment, however, is far from satisfaction, and the mechanisms remain unclear. Using immunohistochemistry and western blotting analysis, we found α-synuclein (SNCA) was significantly up-regulated in the spinal caudal segment of rats subjected to spinal cord transection at 3 days post-operation. Moreover, the role of SNCA on neuronal growth and apoptosis in vitro was determined by using overexpressing and interfering SNCA recombined plasmid vectors, and the underlying mechanism was detected by QRT-PCR and western blotting. Spinal neurons transfected with SNCA-shRNA lentivirus gave rise to an optimal neuronal survival, while it results in cell apoptosis in SNCA-ORF group. In molecular level, SNCA silence induced the up-regulation of CNTF and down-regulation of Caspase7/9. Together, endogenous SNCA plays a crucial role in spinal neuronal survival, in which the underlying mechanism may be linked to the regulation both apoptotic genes (Caspase7/9) and CNTF. The present findings therefore provide novel insights into the role of SNCA in spinal cord and associated mechanism, which may provide novel cue for the treatment of SCI in future clinic trials.

Indexed as

Apoptosisalpha-SynucleinAnimalsAntigens, NuclearCell SurvivalCiliary Neurotrophic FactorDisease Models, AnimalFemaleLentivirusNerve Growth FactorsNerve Tissue ProteinsNeuronsOpen Reading FramesRats, Sprague-DawleyRNA, Small InterferingSpinal Cordalpha-SynucleinAntigens, NuclearCiliary Neurotrophic FactorNerve Growth FactorsNerve Tissue ProteinsRbfox3 protein, ratRNA, Small InterferingAlpha-synucleinApoptotic geneCiliary neurotrophic factorPrimary spinal neuronsSpinal cord transection

Identifiers

PMID27581683
PMCPMC11482142
OpenAlexW2509665554

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.