ArticleBMC plant biology2016
RNAi-mediated down-regulation of the expression of OsFAD2-1: effect on lipid accumulation and expression of lipid biosynthetic genes in the rice grain.
Article in BMC plant biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 49 citations in OpenAlex.
- Negative Regulators of Rice Agronomic Traits: Functional Insights and Applications in Genome Editing-Based Breeding.Plant biotechnology journal · 2026Review
- Metabolic engineering of cereal lipids: from omega-3 fatty acids to wax esters and pheromones.Advanced biotechnology · 2026Review
- Genetic Regulation and Breeding Application of Medium-Chain Fatty Acids Metabolism in Rice.Biology · 2025Review
- Lipidomics-based association study reveals genomic signatures of anti-cancer qualities of pigmented rice sprouts.Frontiers in plant science · 2025Article
- Genetic Loci Mining and Candidate Gene Analysis for Determining Fatty Acid Composition in Rice.Genes · 2024Article
- Upstream regulator of genomic imprinting in rice endosperm is a small RNA-associated chromatin remodeler.Nature communications · 2024Article
- Biological Roles of Lipids in Rice.International journal of molecular sciences · 2024Review
- OsCRLK2, a Receptor-Like Kinase Identified by QTL Analysis, is Involved in the Regulation of Rice Quality.Rice (New York, N.Y.) · 2024Article
- Rice lipases: a conundrum in rice bran stabilization: a review on their impact and biotechnological interventions.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
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- Facile analysis of rice bran oil to compare free unsaturated fatty acid compositions of parental and hybrid rice lines.Journal of the American Oil Chemists' Society · 2022Article
- Evidence and Impacts of Nanoplastic Accumulation on Crop Grains.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Natural Variation of Fatty Acid Desaturase Gene Affects Linolenic Acid Content and Starch Pasting Viscosity in Rice Grains.International journal of molecular sciences · 2022Article
- qFC6, a major gene for crude fat content and quality in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Mutations ofPlants (Basel, Switzerland) · 2020Article
- Article
- Genome-wide analysis of fatty acid desaturase genes in rice (Oryza sativa L.).Scientific reports · 2019Article
- Fertility of Pedicellate Spikelets in Sorghum Is Controlled by a Jasmonic Acid Regulatory Module.International journal of molecular sciences · 2019Article
- Lipid metabolites in seeds of diverse Gossypium accessions: molecular identification of a high oleic mutant allele.Planta · 2017Article
- Powering Genome Editing in Rice by Harnessing Promising Gene Resources: A Comprehensive Roadmap.Physiologia plantarumReview
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe bran from polished rice grains can be used to produce rice bran oil (RBO). High oleic (HO) RBO has been generated previously through RNAi down-regulation of OsFAD2-1. HO-RBO has higher oxidative stability and could be directly used in the food industry without hydrogenation, and is hence free of trans fatty acids. However, relative to a classic oilseed, lipid metabolism in the rice grain is poorly studied and the genetic alteration in the novel HO genotype remains unexplored.
resultsHere, we have undertaken further analysis of role of OsFAD2-1 in the developing rice grain. The use of Illumina-based NGS transcriptomics analysis of developing rice grain reveals that knockdown of Os-FAD2-1 gene expression was accompanied by the down regulation of the expression of a number of key genes in the lipid biosynthesis pathway in the HO rice line. A slightly higher level of oil accumulation was also observed in the HO-RBO.
conclusionProminent among the down regulated genes were those that coded for FatA, LACS, SAD2, SAD5, caleosin and steroleosin. It may be possible to further increase the oleic acid content in rice oil by altering the expression of the lipid biosynthetic genes that are affected in the HO line.
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