ArticleParasites & vectors2016
Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Noninvasive Biological Samples to Detect and Diagnose Infections due to Trypanosomatidae Parasites: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2020Pooled it
- Hidden menace: Understanding the devastating consequences of dourine disease in horses.Open veterinary journal · 2025Review
- Article
- Mechanisms of life cycle simplification in African trypanosomes.Nature communications · 2024Article
- Molecular identification of new Trypanosoma evansi type non-A/B isolates from buffaloes and cattle in Indonesia.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2024Article
- European Inter-Laboratory Proficiency Test for Dourine Antibody Detection Using the Complement Fixation Test.Veterinary sciences · 2023Article
- DOES TRYPANOSOMA EVANSI HAVE THE MAXICIRCLE GENE, OR CAN TRYPANOSOMA EQUIPERDUM BE ISOLATED FROM BOVINES?The Journal of parasitology · 2023Article
- Molecular Analysis of Trypanosome Infections in Algerian Camels.Acta parasitologica · 2022Article
- A review on the diagnosis of animal trypanosomoses.Parasites & vectors · 2022Review
- Biological activity evaluations of chemical constituents derived from Mongolian medicinal forage plants and their applications in combating infectious diseases and addressing health problems in humans and livestock.Journal of natural medicines · 2021Review
- Pathology of female mice experimentally infected with an in vitro cultured strain of Trypanosoma equiperdum.The Journal of veterinary medical science · 2021Article
- MonomorphicMicrobial genomics · 2021Article
- Immunohistochemical phenotyping of macrophages and T lymphocytes infiltrating in peripheral nerve lesions of dourine-affected horses.The Journal of veterinary medical science · 2020Article
- Humoral immune response and delayed-type hypersensitivity in rabbits infected with Trypanosoma equiperdum.Scientific reports · 2020Article
- Nationwide serological surveillance of non-tsetse-transmitted horse trypanosomoses in Mongolia.Parasite epidemiology and control · 2020Article
- Sustainable Elimination (Zero Cases) of Sleeping Sickness: How Far Are We from Achieving This Goal?Pathogens (Basel, Switzerland) · 2019Review
- Histopathological lesions in reproductive organs, distal spinal cord and peripheral nerves of horses naturally infected with Trypanosoma equiperdum.BMC veterinary research · 2019Article
- Equine trypanosomosis: enigmas and diagnostic challenges.Parasites & vectors · 2019Review
- Draft Genome Sequence ofMicrobiology resource announcements · 2019Article
- The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia.Parasitology research · 2018Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrypanosoma equiperdum causes dourine via sexual transmission in Equidae. T. equiperdum is classified under the subgenus Trypanozoon along with the T. brucei sspp. and T. evansi; however, the species classification of Trypanozoon remains a controversial topic due to the limited number of T. equiperdum reference strains. In addition, it is possible that some were misclassified T. evansi strains. Thus, there is a strong need for a new T. equiperdum strain directly isolated from the genital mucosa of a horse with a clinically- and parasitologically-confirmed dourine infection.
methodsTrypanosomes isolated from the urethral tract of a stallion with suspected dourine, were directly cultivated using soft agarose media at 37 °C in 5 % CO2. For molecular characterization, 18S ribosomal RNA (rRNA) gene, the internal transcribed spacer (ITS) and 8 maxicircle DNA regions were amplified by a PCR and their sequences were determined. To analyze the ratio of the kinetoplastic/akinetoplastic population, the kinetoplasts and the nuclei of trypanosomes were subjected to Hoechst staining and observed by fluorescence microscopy.
resultsIn addition to the clinical symptoms and the molecular diagnosis, this stallion was definitively diagnosed with dourine by the detection of trypanosomes in the urethral mucosa. These results strongly suggested that the isolated trypanosome was true T. equiperdum. T. equiperdum isolated from the urethral tract was adapted in vitro using soft agarose media. Based on the results of a phylogenetic analysis of 18S rRNA and ITS, this T. equiperdum isolate was classified into the Trypanozoon clade. In a PCR of the maxicircle DNA region, only NADH-dehydrogenase subunits 4 and 5 was amplified. Clear kinetoplasts were observed in most of the T. equiperdum isolates. In contrast, most culture-adapted T. equiperdum were of the akinetoplastic form.
conclusionWe concluded that our isolated trypanosome was the first confirmed case of T. equiperdum in Mongolia and named it "T. equiperdum IVM-t1". T. equiperdum IVM-t1 was well adapted and propagated in soft agarose media, which indicates that this culture method is useful for isolation of T. equiperdum from horses with dourine.
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