ArticleParasites & vectors2016
Ecological scenario and Trypanosoma cruzi DTU characterization of a fatal acute Chagas disease case transmitted orally (Espírito Santo state, Brazil).
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 2 of them syntheses that pooled it.
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Who cites it
44 citing papers in PubMed, 2 syntheses or guidelines pooled it, 64 citations in OpenAlex.
- Triatomine Fauna and Natural Infection by Trypanosoma (Schizotrypanum) cruzi (Kinetoplastea: Trypanosomatidae) in the Caribbean Region of Panama, Colombia, and Venezuela: A Systematic Review.Neotropical entomology · 2025Pooled it
- Technological advances in the serological diagnosis of Chagas disease in dogs and cats: a systematic review.Parasites & vectors · 2022Pooled it
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- Landscape modeling as a surveillance strategy for Trypanosoma cruzi vectors in Brazil.Parasites & vectors · 2026Article
- Who Feeds theMicroorganisms · 2026Article
- Unveiling the ecology and spatial dynamics of Trypanosoma cruzi, its DTUs and Triatoma vitticeps in the Atlantic Forest of south-eastern Espírito Santo State, Brazil.PLoS neglected tropical diseases · 2026Article
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- A comprehensive genetic and phylogenetic study of Trypanosoma spp. in bats and sand flies from shared habitats in Thailand.Parasites & vectors · 2025Article
- Trypanosoma dionisii in China: ecology and tentative epidemiology.Infectious diseases of poverty · 2025Article
- Targeting Cysteine Proteases and their Inhibitors to Combat Trypanosomiasis.Current medicinal chemistry · 2024Review
- The MiteMicroorganisms · 2023Article
- Deep serological profiling of the Trypanosoma cruzi TSSA antigen reveals different epitopes and modes of recognition by Chagas disease patients.PLoS neglected tropical diseases · 2023Article
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- Molecular detection ofParasitology · 2022Article
- Molecular characterization ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- "Visiting old, learn new": taxonomical overview of chiropteran trypanosomes from the morphology to the genes.Parasitology research · 2022Review
- Article
- Different profiles and epidemiological scenarios: past, present and future.Memorias do Instituto Oswaldo Cruz · 2022Article
- Chagas disease in the context of the 2030 agenda: global warming and vectors.Memorias do Instituto Oswaldo Cruz · 2022Article
- Trypanosomatid Richness Among Rats, Opossums, and Dogs in the Caatinga Biome, Northeast Brazil, a Former Endemic Area of Chagas Disease.Frontiers in cellular and infection microbiology · 2022Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrypanosoma cruzi infection via oral route results in outbreaks or cases of acute Chagas disease (ACD) in different Brazilian regions and poses a novel epidemiological scenario. In the Espírito Santo state (southeastern Brazil), a fatal case of a patient with ACD led us to investigate the enzootic scenario to avoid the development of new cases. At the studied locality, Triatoma vitticeps exhibited high T. cruzi infection rates and frequently invaded residences.
methodsSylvatic and domestic mammals in the Rio da Prata locality, where the ACD case occurred, and in four surrounding areas (Baia Nova, Buenos Aires, Santa Rita and Todos os Santos) were examined and underwent parasitological and serological tests. Triatomines were collected for a fecal material exam, culturing and mini-exon gene molecular characterization, followed by RFLP-PCR of H3/Alul. Paraffin-embedded cardiac tissue of a patient was washed with xylene to remove paraffin and DNA was extracted using the phenol-chloroform method. For genotype characterization, PCR was performed to amplify the 1f8, GPI and 18S rRNA genes. In the case of V7V8 SSU rRNA, the PCR products were molecularly cloned. PCR products were sequenced and compared to sequences in GenBank. Phylogenetic analysis using maximum likelihood method with 1000 bootstrap replicates was performed.
resultsNone of the animals showed positive hemocultures. Three rodents and two dogs showed signs of infection, as inferred from borderline serological titers. T. vitticeps was the only triatomine species identified and showed T. cruzi infection by DTUs TcI and TcIV. The analysis of cardiac tissue DNA showed mixed infection by T. cruzi (DTUs I, II, III and IV) and Trypanosoma dionisii.
conclusionsEach case or outbreak of ACD should be analyzed as a particular epidemiological occurrence. The results indicated that mixed infections in humans may play a role in pathogenicity and may be more common than is currently recognized. Direct molecular characterization from biological samples is essential because this procedure avoids parasite selection. T. dionisii may under certain and unknown circumstances infect humans. The distribution of T. cruzi DTUS TcIII and TcIV in Brazilian biomes is broader than has been assumed to date.
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