ArticleScientific reports2016
Conversion between two conformational states of KaiC is induced by ATP hydrolysis as a trigger for cyanobacterial circadian oscillation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 41 citations in OpenAlex.
- Size-exclusion chromatography-small-angle neutron scattering system optimized for an instrument with medium neutron flux.Journal of applied crystallography · 2025Article
- Programming scheduled self-assembly of circadian materials.Nature communications · 2025Article
- The cyanobacterial circadian clock.Npj biological timing and sleep · 2025Review
- KaiC family ATPases in the nonheterocystous nitrogen-fixing cyanobacterium Leptolyngbya boryana.Scientific reports · 2024Article
- Article
- Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie Brilliant Blue G-250 and R-250 dyes.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- Role of the reaction-structure coupling in temperature compensation of the KaiABC circadian rhythm.PLoS computational biology · 2022Article
- Coupling of distant ATPase domains in the circadian clock protein KaiC.Nature structural & molecular biology · 2022Article
- Highly sensitive tryptophan fluorescence probe for detecting rhythmic conformational changes of KaiC in the cyanobacterial circadian clock system.The Biochemical journal · 2022Article
- Elucidation of master allostery essential for circadian clock oscillation in cyanobacteria.Science advances · 2022Article
- Overall structure of fully assembled cyanobacterial KaiABC circadian clock complex by an integrated experimental-computational approach.Communications biology · 2022Article
- Mechanism of autonomous synchronization of the circadian KaiABC rhythm.Scientific reports · 2021Article
- Article
- Cooperative Binding of KaiB to the KaiC Hexamer Ensures Accurate Circadian Clock Oscillation in Cyanobacteria.International journal of molecular sciences · 2019Article
- Pressure accelerates the circadian clock of cyanobacteria.Scientific reports · 2019Article
- Conformational rearrangements of the C1 ring in KaiC measure the timing of assembly with KaiB.Scientific reports · 2018Article
- Single-molecular and ensemble-level oscillations of cyanobacterial circadian clock.Biophysics and physicobiology · 2018Article
- Role of ATP Hydrolysis in Cyanobacterial Circadian Oscillator.Scientific reports · 2017Article
- Period Robustness and Entrainability of the Kai System to Changing Nucleotide Concentrations.Biophysical journal · 2017Article
- A thermodynamically consistent model of the post-translational Kai circadian clock.PLoS computational biology · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cyanobacterial circadian oscillator can be reconstituted in vitro by mixing three clock proteins, KaiA, KaiB and KaiC, with ATP. KaiC is the only protein with circadian rhythmic activities. In the present study, we tracked the complex formation of the three Kai proteins over time using blue native (BN) polyacrylamide gel electrophoresis (PAGE), in which proteins are charged with the anionic dye Coomassie brilliant blue (CBB). KaiC was separated as three bands: the KaiABC complex, KaiC hexamer and KaiC monomer. However, no KaiC monomer was observed using gel filtration chromatography and CBB-free native PAGE. These data indicate two conformational states of KaiC hexamer and show that the ground-state KaiC (gs-KaiC) is stable and competent-state KaiC (cs-KaiC) is labile and degraded into monomers by the binding of CBB. Repeated conversions from gs-KaiC to cs-KaiC were observed over 24 h using an in vitro reconstitution system. Phosphorylation of KaiC promoted the conversion from gs-KaiC to cs-KaiC. KaiA sustained the gs-KaiC state, and KaiB bound only cs-KaiC. An E77Q/E78Q-KaiC variant that lacked N-terminal ATPase activity remained in the gs-KaiC state. Taken together, ATP hydrolysis induces the formation of cs-KaiC and promotes the binding of KaiB, which is a trigger for circadian oscillations.
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