ArticlePlanta2017
Characterization of the cinnamoyl-CoA reductase (CCR) gene family in Populus tomentosa reveals the enzymatic active sites and evolution of CCR.
Article in Planta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
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- Comprehensive analysis of cinnamoyl-CoA reductase (CCR) gene family in wheat: implications for lignin biosynthesis and stress responses.BMC plant biology · 2025Article
- Genome-wide identification and expression pattern analysis of the cinnamoyl-CoA reductase gene family in flax (Linum usitatissimum L.).BMC genomics · 2025Article
- Identification and expression analysis of cinnamyl CoA reductase gene family and function ofFrontiers in plant science · 2025Article
- Genome-wide characterization and stress-responsive expression analysis of the cinnamoyl-CoA reductase gene family in soybean.Frontiers in plant science · 2025Article
- Simultaneous Down-Regulation of Dominant Cinnamoyl CoA Reductase and Cinnamyl Alcohol Dehydrogenase Dramatically Altered Lignin Content in Mulberry.Plants (Basel, Switzerland) · 2024Article
- Identification of a cinnamoyl-CoA reductase from Cinnamomum cassia involved in trans-cinnamaldehyde biosynthesis.Planta · 2024Article
- Characterization, Expression Profiling, and Biochemical Analyses of theInternational journal of molecular sciences · 2022Article
- Comparative Genomic Analysis ofInternational journal of molecular sciences · 2022Article
- Genome-wide identification and characterization ofFrontiers in plant science · 2022Article
- Genome-wide identification, characterization, and genetic diversity ofFrontiers in plant science · 2022Article
- Comparative genomic analysis of thePeerJ · 2022Article
- Systematic Analysis and Biochemical Characterization of the Caffeoyl Shikimate Esterase Gene Family in Poplar.International journal of molecular sciences · 2021Article
- A peroxisomal β-oxidative pathway contributes to the formation of C6-C1 aromatic volatiles in poplar.Plant physiology · 2021Article
- Association of gene expression with syringyl to guaiacyl ratio in sugarcane lignin.Plant molecular biology · 2021Article
- Expression Profiles of Alkaloid-Related Genes across the Organs of Narrow-Leafed Lupin (International journal of molecular sciences · 2021Article
- Article
- Transcriptomic analysis of early fruit development in Chinese white pear (Pyrus bretschneideri Rehd.) and functional identification of PbCCR1 in lignin biosynthesis.BMC plant biology · 2019Article
- Article
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7 authors.
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Abstract
MAIN
conclusionTwo distinct cinnamoyl-coenzyme A reductases (CCRs) from Populus tomentosa were cloned and studied and active sites in CCRs were further identified based on sequence divergence, molecular simulation, and site-directed mutants. Cinnamoyl-coenzyme A (CoA) reductase (CCR) is the first committed gene in the lignin-specific pathway and plays a role in the lignin biosynthesis pathway. In this study, we cloned 11 genes encoding CCR or CCR-like proteins in Populus tomentosa. An enzymatic assay of the purified recombinant P. tomentosa (Pto) CCR and PtoCCR-like proteins indicated that only PtoCCR1 and PtoCCR7 had detectable activities toward hydroxycinnamoyl-CoA esters. PtoCCR1 exhibited specificity for feruloyl-CoA, with no detectable activity for any other hydroxycinnamoyl-CoA esters. However, PtoCCR7 catalyzed p-coumaroyl-CoA, caffeoyl-CoA, feruloyl-CoA, and sinapoyl-CoA with a preference for feruloyl-CoA. Site-directed mutations of selected amino acids divergent between PtoCCR1 and 7, combined with modeling and docking, showed that A132 in CCR7 combined with the catalytic triad might comprise the catalytic center. In CCR7, L192, F155, and H208 were identified as the substrate-binding sites, and site-directed mutations of these amino acids showed obvious changes in catalytic efficiency with respect to both feruloyl-CoA and sinapoyl-CoA. Mutant F155Y exhibited greater catalytic efficiency for sinapoyl-CoA compared with that of wild-type PtoCCR7. Finally, recent genome duplication events provided the foundation for CCR divergence. This study further identified the active sites in CCRs and the evolutionary process of CCRs in terrestrial plants.
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