ArticleJournal of microscopy2017
Preserving elemental content in adherent mammalian cells for analysis by synchrotron-based x-ray fluorescence microscopy.
Article in Journal of microscopy, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 110 citations in OpenAlex.
- X-ray fluorescence microscopy exposure estimates using a single excitation energy.Journal of synchrotron radiation · 2026Article
- Synchrotron XRF Imaging Reveals Manganese Accumulation in the Golgi and Post-Synapses of Neurons and Enhanced Uptake in Astrocytes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Comparison of Freeze-drying and Gradient Dehydration Treatment on X-ray Imaging for Three-dimensional Reconstruction of Early Human Embryonic Brain Samples.Neuroscience bulletin · 2026Article
- ZFT is the major iron and zinc transporter ineLife · 2026Article
- Preservation artifacts in zebrafish X-ray fluorescence imaging.Metallomics : integrated biometal science · 2026Article
- Nanoprobe synchrotron X-ray fluorescence microscopy reveals selenium-rich spherical structure in mouse retinal pigment epithelium.Scientific reports · 2025Article
- 3DCryoHolder: a new open access 3D printable system to store and transport silicon nitride membranes under cryogenic conditions for spectromicroscopy at low temperature.Journal of synchrotron radiation · 2025Article
- A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.Histochemistry and cell biology · 2024Article
- Native Cryo-Correlative Light and Synchrotron X-ray Fluorescence Imaging of Proteins and Essential Metals in Subcellular Neuronal Compartments.Chemical & biomedical imaging · 2024Article
- Correlative single-cell hard X-ray computed tomography and X-ray fluorescence imaging.Communications biology · 2024Article
- In situ label-free X-ray imaging for visualizing the localization of nanomedicines and subcellular architecture in intact single cells.Nature protocols · 2024Review
- Synchrotron X-rays reveal the modes of Fe binding and trace metal storage in the brown algae Laminaria digitata and Ectocarpus siliculosus.Metallomics : integrated biometal science · 2023Article
- Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy.Scientific reports · 2023Article
- Impact of Sample Preparation Methods on Single-Cell X-ray Microscopy and Light Elemental Analysis Evaluated by Combined Low Energy X-ray Fluorescence, STXM and AFM.Molecules (Basel, Switzerland) · 2023Article
- The use of synchrotron X-ray fluorescent imaging to study distribution and content of elements in chemically fixed single cells: a case study using mouse pancreatic beta-cells.Metallomics : integrated biometal science · 2023Article
- Reducing Frost during Cryoimaging Using a Hygroscopic Ice Frame.ACS omega · 2022Article
- Gadolinium and Bio-Metal Association: A Concentration Dependency Tested in a Renal Allograft and Investigated by Micro-Synchrotron XRF.Journal of imaging · 2022Article
- Correlative High-Resolution Imaging of Iron Uptake in Lung Macrophages.Analytical chemistry · 2022Article
- Assessing Cellular Uptake of Exogenous Coenzyme QAntioxidants (Basel, Switzerland) · 2022Article
- Quantitative imaging approaches to understanding biological processing of metal ions.Current opinion in chemical biology · 2022Review
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Trace metals play important roles in biological function, and x-ray fluorescence microscopy (XFM) provides a way to quantitatively image their distribution within cells. The faithfulness of these measurements is dependent on proper sample preparation. Using mouse embryonic fibroblast NIH/3T3 cells as an example, we compare various approaches to the preparation of adherent mammalian cells for XFM imaging under ambient temperature. Direct side-by-side comparison shows that plunge-freezing-based cryoimmobilization provides more faithful preservation than conventional chemical fixation for most biologically important elements including P, S, Cl, K, Fe, Cu, Zn and possibly Ca in adherent mammalian cells. Although cells rinsed with fresh media had a great deal of extracellular background signal for Cl and Ca, this approach maintained cells at the best possible physiological status before rapid freezing and it does not interfere with XFM analysis of other elements. If chemical fixation has to be chosen, the combination of 3% paraformaldehyde and 1.5 % glutaraldehyde preserves S, Fe, Cu and Zn better than either fixative alone. When chemically fixed cells were subjected to a variety of dehydration processes, air drying was proved to be more suitable than other drying methods such as graded ethanol dehydration and freeze drying. This first detailed comparison for x-ray fluorescence microscopy shows how detailed quantitative conclusions can be affected by the choice of cell preparation method.
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