ArticlePLoS genetics2016
Transgenic Expression of the piRNA-Resistant Masculinizer Gene Induces Female-Specific Lethality and Partial Female-to-Male Sex Reversal in the Silkworm, Bombyx mori.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives.Insect science · 2026Review
- Complete transition from chromosomal to cytoplasmic sex determination during prolonged Wolbachia symbiosis.Nature communications · 2026Article
- A W chromosome-derived feminizing piRNA in pyralid moths demonstrates convergent evolution for primary sex determination signals in Lepidoptera.BMC biology · 2025Article
- Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm,Journal of developmental biology · 2024Article
- Multiple independent origins of the female W chromosome in moths and butterflies.Science advances · 2024Article
- CRISPR/Cas9-Mediated Mutagenesis of Sex-SpecificCells · 2022Article
- Co-Expression Network and Time-Course Expression Analyses to Identify Silk Protein Regulatory Factors inInsects · 2022Article
- Manipulating Insect Sex Determination Pathways for Genetic Pest Management: Opportunities and Challenges.Frontiers in bioengineering and biotechnology · 2022Review
- Identification and Characterization of the Masculinizing Function of theInternational journal of molecular sciences · 2021Article
- Evolution of sexual development and sexual dimorphism in insects.Current opinion in genetics & development · 2021Review
- RNase κ promotes robust piRNA production by generating 2',3'-cyclic phosphate-containing precursors.Nature communications · 2021Article
- The Sex Determination Cascade in the Silkworm.Genes · 2021Review
- Gtsf1 is essential for proper female sex determination and transposon silencing in the silkworm, Bombyx mori.PLoS genetics · 2020Article
- Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori.BMC developmental biology · 2020Article
- Regulation of olfactory-based sex behaviors in the silkworm by genes in the sex-determination cascade.PLoS genetics · 2020Article
- Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella.Insect molecular biology · 2020Article
- CRISPR Disruption ofInsects · 2019Article
- A Novel Splice Variant of the Masculinizing GeneBiomolecules · 2019Article
- Dimorphic sperm formation byProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination.PLoS genetics · 2018Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Bombyx mori (B. mori), Fem piRNA originates from the W chromosome and is responsible for femaleness. The Fem piRNA-PIWI complex targets and cleaves mRNAs transcribed from the Masc gene. Masc encodes a novel CCCH type zinc-finger protein and is required for male-specific splicing of B. mori doublesex (Bmdsx) transcripts. In the present study, several silkworm strains carrying a transgene, which encodes a Fem piRNA-resistant Masc mRNA (Masc-R), were generated. Forced expression of the Masc-R transgene caused female-specific lethality during the larval stages. One of the Masc-R strains weakly expressed Masc-R in various tissues. Females heterozygous for the transgene expressed male-specific isoform of the Bombyx homolog of insulin-like growth factor II mRNA-binding protein (ImpM) and Bmdsx. All examined females showed a lower inducibility of vitellogenin synthesis and exhibited abnormalities in the ovaries. Testis-like tissues were observed in abnormal ovaries and, notably, the tissues contained considerable numbers of sperm bundles. Homozygous expression of the transgene resulted in formation of the male-specific abdominal segment in adult females and caused partial male differentiation in female genitalia. These results strongly suggest that Masc is an important regulatory gene of maleness in B. mori.
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