ArticleBiotechnology for biofuels2016
Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Engineering nonconventional yeasts to produce carboxylic acids: Advances and Prospects.Synthetic and systems biotechnology · 2027Review
- Recent progress in microbial succinic acid production: integrating genome engineering and fermentation strategies.World journal of microbiology & biotechnology · 2026Review
- Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production inSynthetic and systems biotechnology · 2026Article
- Development of a CRISPR/Cas12a genome editing toolbox inSynthetic and systems biotechnology · 2026Article
- Improving succinic acid production by tuning polysulfides metabolism in Yarrowia lipolytica.Microbial cell factories · 2025Article
- Microbial Bioprocessing of Food and Agro-Industrial Residues for a Sustainable Circular Economy.Indian journal of microbiology · 2025Review
- Succinic Acid Production from Monosaccharides and Woody and Herbaceous Plant Hydrolysates Using Metabolically EngineeredFood technology and biotechnology · 2025Article
- Engineering Yarrowia lipolytica to Enhance the Production of Malonic Acid via Malonyl-CoA Pathway at High Titer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Glycerol bioconversion to biofuel and value-added products by yeasts.FEMS yeast research · 2025Review
- Harnessing yeasts for sustainable succinic acid production: advances in metabolic engineering and biorefinery integration.FEMS yeast research · 2025Review
- Engineering of itaconic acid pathway via co-localization of CadA and AcnA in recombinant Escherichia coli.Biotechnology letters · 2024Article
- Recent advances in bio-based production of top platform chemical, succinic acid: an alternative to conventional chemistry.Biotechnology for biofuels and bioproducts · 2024Review
- Succinic acid - A run-through of the latest perspectives of production from renewable biomass.Heliyon · 2024Review
- Mitochondrial membrane transporters as attractive targets for the fermentative production of succinic acid from glycerol in Saccharomyces cerevisiae.FEMS yeast research · 2024Article
- Proteomes reveal metabolic capabilities ofmSystems · 2023Article
- Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica.Nature communications · 2023Article
- A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering.Communications biology · 2023Article
- Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects.Foods (Basel, Switzerland) · 2023Review
- A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering.Research square · 2023Article
- Modelling of the Citric Acid Production from Crude Glycerol by Wild-TypeLife (Basel, Switzerland) · 2022Article
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIntegrating waste management with fuels and chemical production is considered to address the food waste problem and oil crisis. Approximately, 600 million tonnes crude glycerol is produced from the biodiesel industry annually, which is a top renewable feedstock for succinic acid production. To meet the increasing demand for succinic acid production, the development of more efficient and cost-effective production methods is urgently needed. Herein, we have proposed a new strategy for integration of both biodiesel and SA production in a biorefinery unit by construction of an aerobic yeast Yarrowia lipolytica with a deletion in the gene coding succinate dehydrogenase subunit 5.
resultsRobust succinic acid production by an engineered yeast Y. lipolytica from crude glycerol without pre-treatment was demonstrated. Diversion of metabolic flow from tricarboxylic acid cycle led to the success in generating a succinic acid producer Y. lipolytica PGC01003. The fermentation media and conditions were optimized, which resulted in 43 g L(-1) succinic acid production from crude glycerol. Using the fed-batch strategy in 2.5 L fermenter, up to 160 g L(-1) SA was yielded, indicating the great industrial potential.
conclusionsInactivation of SDH5 in Y. lipolytica Po1f led to succinic acid accumulation and secretion significantly. To our best knowledge, this is the highest titer obtained in fermentation on succinic acid production. In addition, the performance of batch and fed-batch fermentation showed high tolerance and yield on biodiesel by-product crude glycerol. All these results indicated that PGC01003 is a promising microbial factorial cell for the highly efficient strategy solving the environmental problem in connection with the production of value-added product.
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