Evidence map›Paper›PMID 27579043›Full record

ArticleStem cells international2016

Investigation of the Cell Surface Proteome of Human Periodontal Ligament Stem Cells.

Jimin Xiong, Danijela Menicanin, Peter S Zilm, Victor Marino, P Mark Bartold, Stan Gronthos

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
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  4. Article
  5. Review
  6. Article
  7. Review
  8. Mineralization Induction of Gingival Fibroblasts and Construction of a Sandwich Tissue-Engineered Complex for Repairing Periodontal Defects.Medical science monitor : international medical journal of experimental and clinical research · 2018
    Article
  9. Article
  10. Review
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 3 countries.

Jimin XiongColgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, SA 50052, Australia; Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing 1000443, China.
Danijela MenicaninSchool of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 50054, Australia.
Peter S ZilmColgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, SA 50052, Australia; Oral Microbiology, School of Dentistry, University of Adelaide, Adelaide, SA 50055, Australia.ORCID 0000-0001-7554-9717
Victor MarinoColgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, SA 50052, Australia.
P Mark BartoldColgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, SA 50052, Australia.ORCID 0000-0002-5695-3877
Stan GronthosMesenchymal Stem Cell Laboratory, School of Medicine, Faculty of Health Sciences, University of Adelaide, Adelaide, SA 50056, Australia; South Australian Health and Medical Research Institute, Adelaide, SA 5000, Australia.ORCID 0000-0002-6225-3084
Australian Dental Association · AUSouth Australian Health and Medical Research Institute · AUSouth Australia Pathology · AUThe University of Adelaide · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study examined the cell surface proteome of human periodontal ligament stem cells (PDLSC) compared to human fibroblasts. Cell surface proteins were prelabelled with CyDye before processing to extract the membrane lysates, which were separated using 2D electrophoresis. Selected differentially expressed protein "spots" were identified using Mass spectrometry. Four proteins were selected for validation: CD73, CD90, Annexin A2, and sphingosine kinase 1 previously associated with mesenchymal stem cells. Flow cytometric analysis found that CD73 and CD90 were highly expressed by human PDLSC and gingival fibroblasts but not by keratinocytes, indicating that these antigens could be used as potential markers for distinguishing between mesenchymal cells and epithelial cell populations. Annexin A2 was also found to be expressed at low copy number on the cell surface of human PDLSC and gingival fibroblasts, while human keratinocytes lacked any cell surface expression of Annexin A2. In contrast, sphingosine kinase 1 expression was detected in all the cell types examined using immunocytochemical analysis. These proteomic studies form the foundation to further define the cell surface protein expression profile of PDLSC in order to better characterise this cell population and help develop novel strategies for the purification of this stem cell population.

Identifiers

PMID27579043
PMCPMC4989088
OpenAlexW2493763734

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.