ArticleFrontiers in plant science2016
De novo Sequencing and Transcriptome Analysis of Pinellia ternata Identify the Candidate Genes Involved in the Biosynthesis of Benzoic Acid and Ephedrine.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- A Systematic Review on the Continuous Cropping Obstacles and Control Strategies in Medicinal Plants.International journal of molecular sciences · 2023Pooled it
- Integrative analyses of the transcriptome and metabolome of Huzhang (Pinellia pedatisecta Schott) uncover the molecular genetic basis of its medicinal properties.BMC genomics · 2026Article
- Identification and functional verification of key genes involved in alkaloid biosynthesis inFrontiers in plant science · 2026Article
- Principal Component Analysis to Assess the Changes of Yield and Quality inInternational journal of molecular sciences · 2022Article
- Transcriptome Analysis Reveals an Essential Role of Exogenous Brassinolide on the Alkaloid Biosynthesis Pathway in Pinellia Ternata.International journal of molecular sciences · 2022Article
- Involvement ofFrontiers in plant science · 2022Article
- Dynamic analysis of physiological indices and transcriptome profiling revealing the mechanisms of the allelopathic effects of phenolic acids onFrontiers in plant science · 2022Article
- Rhizosphere microbial diversity in rhizosphere of3 Biotech · 2021Article
- Parallel analysis of global garlic gene expression and alliin content following leaf wounding.BMC plant biology · 2021Article
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- De novo RNA sequencing analysis of Aeluropus littoralis halophyte plant under salinity stress.Scientific reports · 2020Article
- Cross-Species Annotation of Expressed Genes and Detection of Different Functional Gene Modules Between 10 Cold- and 10 Hot-Propertied Chinese Herbal Medicines.Frontiers in genetics · 2020Article
- Full-length transcriptome analysis of shade-induced promotion of tuber production in Pinellia ternata.BMC plant biology · 2019Article
- De novo sequencing and transcriptome assembly of Arisaema heterophyllum Blume and identification of genes involved in isoflavonoid biosynthesis.Scientific reports · 2018Article
- Inhibitory effect and mechanism of Tagetes erecta L. fungicide on Fusarium oxysporum f. sp. niveum.Scientific reports · 2017Article
- Researches on Transcriptome Sequencing in the Study of Traditional Chinese Medicine.Evidence-based complementary and alternative medicine : eCAM · 2017Review
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6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe medicinal herb, Pinellia ternata, is purported to be an anti-emetic with analgesic and sedative effects. Alkaloids are the main biologically active compounds in P. ternata, especially ephedrine that is a phenylpropylamino alkaloid specifically produced by Ephedra and Catha edulis. However, how ephedrine is synthesized in plants is uncertain. Only the phenylalanine ammonia lyase (PAL) and relevant genes in this pathway have been characterized. Genomic information of P. ternata is also unavailable.
resultsWe analyzed the transcriptome of the tuber of P. ternata with the Illumina HiSeq™ 2000 sequencing platform. 66,813,052 high-quality reads were generated, and these reads were assembled de novo into 89,068 unigenes. Most known genes involved in benzoic acid biosynthesis were identified in the unigene dataset of P. ternata, and the expression patterns of some ephedrine biosynthesis-related genes were analyzed by reverse transcription quantitative real-time PCR (RT-qPCR). Also, 14,468 simple sequence repeats (SSRs) were identified from 12,000 unigenes. Twenty primer pairs for SSRs were randomly selected for the validation of their amplification effect.
conclusionRNA-seq data was used for the first time to provide a comprehensive gene information on P. ternata at the transcriptional level. These data will advance molecular genetics in this valuable medicinal plant.
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