ArticleMolecular plant pathology2017
Deep sequencing of mycovirus-derived small RNAs from Botrytis species.
Article in Molecular plant pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 54 citations in OpenAlex.
- Botrytis cinerea Protoplasts Production and Inoculation.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Identification of Mycoviral Infections.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A newly established reverse genetic system for a circular RNA virus reveals new requirements for infection and its biocontrol potential.Science advances · 2026Article
- New viral sequences and endogenous viral elements (EVE) in world-wide populations of Trioza erytreae, the African citrus psyllid.Virus research · 2026Article
- The Viruses ofViruses · 2025Article
- Reexamining the Mycovirome ofViruses · 2024Article
- Mycologists and Virologists Align: ProposingViruses · 2024Review
- Determination of the Mycovirome of a Necrotrophic Fungus.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Construction of a Mycoviral Infectious Clone for Reverse Genetics in Botrytis cinerea.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The First Virome of a Russian Vineyard.Plants (Basel, Switzerland) · 2023Article
- Divergent RNA viruses infecting sea lice, major ectoparasites of fish.PLoS pathogens · 2023Article
- RNA interference ofFrontiers in microbiology · 2023Article
- Viral cross-class transmission results in disease of a phytopathogenic fungus.The ISME journal · 2022Article
- Interference of Small RNAs inJournal of fungi (Basel, Switzerland) · 2022Article
- Similar Characteristics of siRNAs of Plant Viruses Which Replicate in Plant and Fungal Hosts.Biology · 2022Article
- The Mycovirome in a Worldwide Collection of the Brown Rot FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Construction and Characterization of a Botrytis Virus F Infectious Clone.Journal of fungi (Basel, Switzerland) · 2022Article
- Genome Characterization and Phylogenetic Analysis of a Novel Endornavirus That Infects Fungal PathogenViruses · 2022Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing is an ancient regulatory mechanism operating in all eukaryotic cells. In fungi, it was first discovered in Neurospora crassa, although its potential as a defence mechanism against mycoviruses was first reported in Cryphonectria parasitica and, later, in several fungal species. There is little evidence of the antiviral potential of RNA silencing in the phytopathogenic species of the fungal genus Botrytis. Moreover, little is known about the RNA silencing components in these fungi, although the analysis of public genome databases identified two Dicer-like genes in B. cinerea, as in most of the ascomycetes sequenced to date. In this work, we used deep sequencing to study the virus-derived small RNA (vsiRNA) populations from different mycoviruses infecting field isolates of Botrytis spp. The mycoviruses under study belong to different genera and species, and have different types of genome [double-stranded RNA (dsRNA), (+)single-stranded RNA (ssRNA) and (-)ssRNA]. In general, vsiRNAs derived from mycoviruses are mostly of 21, 20 and 22 nucleotides in length, possess sense or antisense orientation, either in a similar ratio or with a predominance of sense polarity depending on the virus species, have predominantly U at their 5' end, and are unevenly distributed along the viral genome, showing conspicuous hotspots of vsiRNA accumulation. These characteristics reveal striking similarities with vsiRNAs produced by plant viruses, suggesting similar pathways of viral targeting in plants and fungi. We have shown that the fungal RNA silencing machinery acts against the mycoviruses used in this work in a similar manner independent of their viral or fungal origin.
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