ArticleScientific reports2016
Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Electrically switchable dual-focus metalens for depth-extended in vivo photoacoustic imaging of ophthalmic vascular disease.Light, science & applications · 2026Article
- Integrated two-photon and photoacoustic microscopy for single-cell neurometabolic imaging.Nature communications · 2026Article
- A comprehensive review of high-performance photoacoustic microscopy systems.Photoacoustics · 2025Review
- Integrated two-photon and optoacoustic microscopy for functional neuroimaging.Scientific reports · 2025Article
- Transparent microfiber Fabry-Perot ultrasound sensor with needle-shaped focus for multiscale photoacoustic imaging.Photoacoustics · 2023Article
- Visualizing vasculature and its response to therapy in the tumor microenvironment.Theranostics · 2023Review
- Photoacoustic imaging for microcirculation.Microcirculation (New York, N.Y. : 1994) · 2022Review
- The emerging role of photoacoustic imaging in clinical oncology.Nature reviews. Clinical oncology · 2022Review
- Methods and applications of full-field optical coherence tomography: a review.Journal of biomedical optics · 2022Review
- Integrating photoacoustic microscopy with other imaging technologies for multimodal imaging.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Photoacoustic Neuroimaging - Perspectives on a Maturing Imaging Technique and its Applications in Neuroscience.Frontiers in neuroscience · 2021Review
- Pushing the boundaries of optoacoustic microscopy by total impulse response characterization.Nature communications · 2020Article
- Integrating photoacoustic microscopy, optical coherence tomography, OCT angiography, and fluorescence microscopy for multimodal imaging.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- A review of clinical photoacoustic imaging: Current and future trends.Photoacoustics · 2019Review
- Review on practical photoacoustic microscopy.Photoacoustics · 2019Review
- Optical coherence tomography-guided dynamic focusing for combined optical and mechanical scanning multimodal photoacoustic microscopy.Journal of biomedical optics · 2019Article
- The integrated high-resolution reflection-mode photoacoustic and fluorescence confocal microscopy.Photoacoustics · 2019Article
- Integrated multimodal photoacoustic microscopy with OCT- guided dynamic focusing.Biomedical optics express · 2019Article
- Direct mineralogical imaging of economic ore and rock samples with multi-modal nonlinear optical microscopy.Scientific reports · 2018Article
- Imaging Collagen in Scar Tissue: Developments in Second Harmonic Generation Microscopy for Biomedical Applications.International journal of molecular sciences · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to obtain comprehensive structural and functional information from intact biological tissue in vivo is highly desirable for many important biomedical applications, including cancer and brain studies. Here, we developed a fully integrated multimodal microscopy that can provide photoacoustic (optical absorption), two-photon (fluorescence), and second harmonic generation (SHG) information from tissue in vivo, with intrinsically co-registered images. Moreover, using a delicately designed optical-acoustic coupling configuration, a high-frequency miniature ultrasonic transducer was integrated into a water-immersion optical objective, thus allowing all three imaging modalities to provide a high lateral resolution of ~290 nm with reflection-mode imaging capability, which is essential for studying intricate anatomy, such as that of the brain. Taking advantage of the complementary and comprehensive contrasts of the system, we demonstrated high-resolution imaging of various tissues in living mice, including microvasculature (by photoacoustics), epidermis cells, cortical neurons (by two-photon fluorescence), and extracellular collagen fibers (by SHG). The intrinsic image co-registration of the three modalities conveniently provided improved visualization and understanding of the tissue microarchitecture. The reported results suggest that, by revealing complementary tissue microstructures in vivo, this multimodal microscopy can potentially facilitate a broad range of biomedical studies, such as imaging of the tumor microenvironment and neurovascular coupling.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.