ArticleScientific reports2016
Sensitive detection of miRNA by using hybridization chain reaction coupled with positively charged gold nanoparticles.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- In Situ Single-Particle LSPR Biosensor for Amplification-Free Detection of miR-451 via Dark-Field Scattering Spectroscopy.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Paper Matters: Technical Evaluation of Paper-Based Substrates for Enhanced Preconcentration of Biomolecules in Liquid Biopsy Diagnostics.Analytical chemistry · 2025Article
- Review
- Polydopamine functionalized dendritic fibrous silica nanoparticles as a generic platform for nucleic acid-based biosensing.Mikrochimica acta · 2024Article
- Label-Free miRNA-21 Analysis Based on Strand Displacement and Terminal Deoxynucleotidyl Transferase-Assisted Amplification Strategy.Biosensors · 2022Article
- Epigenetic Landscape of Liquid Biopsy in Colorectal Cancer.Frontiers in cell and developmental biology · 2021Review
- Hybridization Chain Reactions Targeting the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2).International journal of molecular sciences · 2020Article
- Physical and chemical template-blocking strategies in the exponential amplification reaction of circulating microRNAs.Analytical and bioanalytical chemistry · 2020Article
- Isothermal digital detection of microRNAs using background-free molecular circuit.Science advances · 2020Article
- A nanobiosensor based on graphene oxide and DNA binding dye for multi-microRNAs detection.Bioscience reports · 2019Article
- Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker.ACS omega · 2019Article
- Catalytic hairpin assembly-assisted lateral flow assay for visual determination of microRNA-21 using gold nanoparticles.Mikrochimica acta · 2019Article
- Research advances in the detection of miRNA.Journal of pharmaceutical analysis · 2019Review
- Gold Nanoparticle-Based Colorimetric Strategies for Chemical and Biological Sensing Applications.Nanomaterials (Basel, Switzerland) · 2019Review
- Aggregation of Gold Nanoparticles Caused in Two Different Ways Involved in 4-Mercaptophenylboronic Acidand Hydrogen Peroxide.Materials (Basel, Switzerland) · 2019Article
- Ultrasensitive detection of miRNA with an antimonene-based surface plasmon resonance sensor.Nature communications · 2019Article
- An organic electrochemical transistor for determination of microRNA21 using gold nanoparticles and a capture DNA probe.Mikrochimica acta · 2018Article
- Ultrasensitive optical biosensor for detection of miRNA-155 using positively charged Au nanoparticles.Scientific reports · 2018Article
- Methods and novel technology for microRNA quantification in colorectal cancer screening.Clinical epigenetics · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Positively charged gold nanoparticles (+)AuNPs can adsorb onto the negatively charged surface of single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA). Herein, long-range dsDNA polymers could form based on the hybridization chain reaction (HCR) of two hairpin probes (H1 and H2) by using miRNA-21 as an initiator. (+)AuNPs could adsorb onto the negatively charged surface of such long-range dsDNA polymers based on the electrostatic adsorption, which directly resulted in the precipitation of (+)AuNPs and the decrease of (+)AuNPs absorption spectra. Under optimal conditions, miRNA-21 detection could be realized in the range of 20 pM-10 nM with a detection limit of 6.8 pM. In addition, (+)AuNPs used here are much more stable than commonly used negatively charged gold nanoparticles ((-)AuNPs) in mixed solution that contained salt, protein or other metal ions. Importantly, the assay could realize the detection of miRNA in human serum samples.
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