ArticleMethods in molecular biology (Clifton, N.J.)2019
Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Applying 3D cultures and high-throughput technologies to study host-pathogen interactions.Frontiers in immunology · 2025Review
- Molecular determinants and heterogeneity underlying host response to EV-A71 infection at single-cell resolution.RNA biology · 2021Article
- Links between Nutrition, Infectious Diseases, and Microbiota: Emerging Technologies and Opportunities for Human-Focused Research.Nutrients · 2020Review
- Intestinal Epithelial Organoids as Tools to Study Epigenetics in Gut Health and Disease.Stem cells international · 2019Review
- GPR35 promotes glycolysis, proliferation, and oncogenic signaling by engaging with the sodium potassium pump.Science signaling · 2019Article
- Interleukin-22 promotes phagolysosomal fusion to induce protection againstProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Designer human tissue: coming to a lab near you.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Morphogenesis and maturation of the embryonic and postnatal intestine.Seminars in cell & developmental biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Intestinal human organoids (iHOs) provide an effective system for studying the intestinal epithelium and its interaction with various stimuli. By using combinations of different signaling factors, human induced pluripotent stem cells (hIPSCs) can be driven to differentiate down the intestinal lineage. Here, we describe the process for this differentiation, including the derivation of hindgut from hIPSCs, embedding hindgut into a pro-intestinal culture system and passaging the resulting iHOs. We then describe how to carry out microinjections to introduce bacteria to the apical side of the intestinal epithelial cells (IECs).
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.