ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2016
Standardization and quality control for high-dimensional mass cytometry studies of human samples.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 70 citations in OpenAlex.
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- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.Methods in molecular biology (Clifton, N.J.) · 2024Review
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- A Hashing-Based Framework for Enhancing Cluster Delineation of High-Dimensional Single-Cell Profiles.Phenomics (Cham, Switzerland) · 2022Article
- CytofIn enables integrated analysis of public mass cytometry datasets using generalized anchors.Nature communications · 2022Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Mass cytometry (CyTOF), a mass spectrometry-based single cell phenotyping technology, allows utilization of over 35 antibodies in a single sample and is a promising tool for translational human immunology studies. Although several analysis tools are available to interpret the complex data sets generated, a robust method for standardization and quality control within and across studies is needed. Here we report an efficient and easily adaptable method to monitor quality of individual samples in human immunology studies and to facilitate reproducible data analysis. Samples to be assessed are spiked with a defined amount of reference peripheral blood mononuclear cells from a healthy donor, derived from a single large blood draw. The presence of known standardized numbers and phenotypic profiles of these reference cells greatly facilitates sample analysis by allowing for: 1) quality control for consistent staining of each antibody in the panel, 2) identification of potential batch effects, and 3) implementation of a robust gating strategy. We demonstrate the utility of this method using peripheral blood and bronchoalveolar lavage samples from HIV
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.