Evidence map›Paper›PMID 27575052›Full record

ArticleThe Journal of organic chemistry2016

HPLC-Assisted Automated Oligosaccharide Synthesis: Implementation of the Autosampler as a Mode of the Reagent Delivery.

Salvatore G Pistorio, Swati S Nigudkar, Keith J Stine, Alexei V Demchenko

Abstract read
In one paragraph

Article in The Journal of organic chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. HPLC-Based Automated Synthesis and Purification of Carbohydrates.Chemistry (Weinheim an der Bergstrasse, Germany) · 2024
    Article
  5. Review
  6. Review
  7. HPLC-Based Automated Synthesis of Glycans in Solution.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022
    Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Machine-Driven Enzymatic Oligosaccharide Synthesis by Using a Peptide Synthesizer.Angewandte Chemie (International ed. in English) · 2018
    Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Salvatore G PistorioDepartment of Chemistry and Biochemistry, University of Missouri-St. Louis , One University Boulevard, St. Louis, Missouri 63121, United States.
Swati S NigudkarDepartment of Chemistry and Biochemistry, University of Missouri-St. Louis , One University Boulevard, St. Louis, Missouri 63121, United States.
Keith J StineDepartment of Chemistry and Biochemistry, University of Missouri-St. Louis , One University Boulevard, St. Louis, Missouri 63121, United States.
Alexei V DemchenkoDepartment of Chemistry and Biochemistry, University of Missouri-St. Louis , One University Boulevard, St. Louis, Missouri 63121, United States.

Funding

Automation of the chemical synthesis of human milk oligosaccharidesR01GM111835 · NIGMS · SAINT LOUIS UNIVERSITY · PI DEMCHENKO, ALEXEI V · 2014 to 2023
$2.5M
Refinement and implementation of the automated oligosaccharide synthesizerU01GM120673 · NIGMS · UNIVERSITY OF MISSOURI-ST. LOUIS · PI DE MEO, CRISTINA, DEMCHENKO, ALEXEI V · 2016 to 2019
$2.2M
NIGMS NIH HHS R01 GM111835NIGMS NIH HHS U01 GM120673
6 · The paper itself

Abstract

The development of a useful methodology for simple, scalable, and transformative automation of oligosaccharide synthesis that easily interfaces with existing methods is reported. The automated synthesis can now be performed using accessible equipment where the reactants and reagents are delivered by the pump or the autosampler and the reactions can be monitored by the UV detector. The HPLC-based platform for automation is easy to setup and adapt to different systems and targets.

Indexed as

AutomationCarbohydrate SequenceChromatography, High Pressure LiquidIndicators and ReagentsOligosaccharidesSpectrophotometry, UltravioletIndicators and ReagentsOligosaccharides

Identifiers

PMID27575052
PMCPMC5496006

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.