ArticleIEEE journal of translational engineering in health and medicine2016
Optical Cryoimaging Reveals a Heterogeneous Distribution of Mitochondrial Redox State in ex vivo Guinea Pig Hearts and Its Alteration During Ischemia and Reperfusion.
Article in IEEE journal of translational engineering in health and medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 17 citations in OpenAlex.
- Endothelial c-Myc and Doxorubicin-Induced Metabolic Alterations: A Multi-Organ Optical Imaging Study.Journal of biophotonics · 2025Article
- Optical Imaging Demonstrates Tissue-Specific Metabolic Perturbations in Mblac1 Knockout Mice.IEEE journal of translational engineering in health and medicine · 2024Article
- Vacuum-assisted tissue embedding for whole-heart imaging.Biomedical optics express · 2023Article
- Cryo-fluorescence micro-optical sectioning tomography for volumetric imaging of various whole organs with subcellular resolution.iScience · 2022Article
- Three-dimensional vascular and metabolic imaging using inverted autofluorescence.Journal of biomedical optics · 2021Article
- Optical Metabolic Imaging of Mitochondrial Dysfunction on HADH Mutant Newborn Rat Hearts.IEEE journal of translational engineering in health and medicine · 2021Article
- The effect of Tmem135 overexpression on the mouse heart.PloS one · 2018Article
- Optical metabolic imaging of irradiated rat heart exposed to ischemia-reperfusion injury.Journal of biomedical optics · 2018Article
- Optical imaging for the assessment of hepatocyte metabolic state in ischemia and reperfusion injuries.Biomedical optics express · 2017Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
Oxidation of substrates to generate ATP in mitochondria is mediated by redox reactions of NADH and FADH2. Cardiac ischemia and reperfusion (IR) injury compromises mitochondrial oxidative phosphorylation. We hypothesize that IR alters the metabolic heterogeneity of mitochondrial redox state of the heart that is only evident in the 3-D optical cryoimaging of the perfused heart before, during, and after IR. The study involved four groups of hearts: time control (TC: heart perfusion without IR), global ischemia (Isch), global ischemia followed by reperfusion (IR) and TC with PCP (a mitochondrial uncoupler) perfusion. Mitochondrial NADH and FAD autofluorescence signals were recorded spectrofluorometrically online in guinea pig ex vivo-perfused hearts in the Langendorff mode. At the end of each specified protocol, hearts were rapidly removed and snap frozen in liquid N2 for later 3-D optical cryoimaging of the mitochondrial NADH, FAD, and NADH/FAD redox ratio (RR). The TC hearts revealed a heterogeneous spatial distribution of NADH, FAD, and RR. Ischemia and IR altered the spatial distribution and caused an overall increase and decrease in the RR by 55% and 64%, respectively. Uncoupling with PCP resulted in the lowest level of the RR (73% oxidation) compared with TC. The 3-D optical cryoimaging of the heart provides novel insights into the heterogeneous distribution of mitochondrial NADH, FAD, RR, and metabolism from the base to the apex during ischemia and IR. This 3-D information of the mitochondrial redox state in the normal and ischemic heart was not apparent in the dynamic spectrofluorometric data.
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