ArticleFrontiers in plant science2016
Isolation of Mesophyll Protoplasts from Mediterranean Woody Plants for the Study of DNA Integrity under Abiotic Stress.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry.Plant methods · 2025Article
- Establishing a reliable protoplast system for grapevine: isolation, transformation, and callus induction.Protoplasma · 2025Article
- Phenotype microarray-based assessment of metabolic variability in plant protoplasts.Plant methods · 2025Article
- Optimization of a rapid, sensitive, and high throughput molecular sensor to measure canola protoplast respiratory metabolism as a means of screening nanomaterial cytotoxicity.Plant methods · 2024Article
- Modern Technologies Provide New Opportunities for Somatic Hybridization in the Breeding of Woody Plants.Plants (Basel, Switzerland) · 2024Review
- Advances inFrontiers in plant science · 2024Article
- A highly efficient protocol for isolation of protoplast from China, Assam and Cambod types of tea plants [Camellia sinensis (L.) O. Kuntze].Plant methods · 2023Article
- The Role of Italy in the Use of Advanced Plant Genomic Techniques on Fruit Trees: State of the Art and Future Perspectives.International journal of molecular sciences · 2023Review
- In Vitro Polyploid Induction of Highbush Blueberry through De Novo Shoot Organogenesis.Plants (Basel, Switzerland) · 2022Article
- First Report on Genome Editing via Ribonucleoprotein (RNP) inInternational journal of molecular sciences · 2022Article
- DNA integrity and ecophysiological responses of Spanish populations of Ulmus glabra to increasing ozone levels.Ecotoxicology (London, England) · 2021Article
- Protoplast: A Valuable Toolbox to Investigate Plant Stress Perception and Response.Frontiers in plant science · 2021Review
- Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants.Plants (Basel, Switzerland) · 2020Review
- In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes.Plants (Basel, Switzerland) · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abiotic stresses have considerable negative impact on Mediterranean plant ecosystems and better comprehension of the genetic control of response and adaptation of trees to global changes is urgently needed. The single cell gel electrophoresis (SCGE) assay could be considered a good estimator of DNA damage in an individual eukaryotic cell. This method has been mainly employed in animal tissues, because the plant cell wall represents an obstacle for the extraction of nuclei; moreover, in Mediterranean woody species, especially in the sclerophyll plants, this procedure can be quite difficult because of the presence of sclerenchyma and hardened cells. On the other hand, these plants represent an interesting material to be studied because of the ability of these plants to tolerate abiotic stress. For instance, holm oak (Quercus ilex L.) has been selected as the model plant to identify critical levels of O3 for Southern European forests. Consequently, a quantitative method for the evaluation of cell injury of leaf tissues of this species is required. Optimal conditions for high-yield nuclei isolation were obtained by using protoplast technology and a detailed description of the method is provided and discussed. White poplar (Populus alba L.) was used as an internal control for protoplast isolation. Such a method has not been previously reported in newly fully developed leaves of holm oak. This method combined with SCGE assay represents a new tool for testing the DNA integrity of leaf tissues in higher plants under stress conditions.
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