ArticleScientific reports2016
Polyurethane scaffolds seeded with CD34(+) cells maintain early stem cells whilst also facilitating prolonged egress of haematopoietic progenitors.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- 3DACS biomaterials science & engineering · 2026Review
- Hyaluronic Acid-Functionalized Highly Porous Polymeric Materials for Stem Cell Culture.Chemistry of materials : a publication of the American Chemical Society · 2025Article
- Review
- Architectural Design of Biomaterial Scaffolds for Hematopoietic Stem Cell Culture.Biomaterials research · 2025Review
- Current status of red blood cell manufacturing in 3D culture and bioreactors.Blood research · 2023Review
- Expansion and differentiation of ex vivo cultured erythroblasts in scalable stirred bioreactors.Biotechnology and bioengineering · 2022Article
- Towards manufactured red blood cells for the treatment of inherited anemia.Haematologica · 2021Review
- 3D Scaffolds to Model the Hematopoietic Stem Cell Niche: Applications and Perspectives.Materials (Basel, Switzerland) · 2021Review
- Differentiation of human induced pluripotent stem cells into erythroid cells.Stem cell research & therapy · 2020Review
- In Vitro Modeling of Non-Solid Tumors: How Far Can Tissue Engineering Go?International journal of molecular sciences · 2020Review
- A three-dimensional in vitro model of erythropoiesis recapitulates erythroid failure in myelodysplastic syndromes.Leukemia · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We describe a 3D erythroid culture system that utilises a porous polyurethane (PU) scaffold to mimic the compartmentalisation found in the bone marrow. PU scaffolds seeded with peripheral blood CD34(+) cells exhibit a remarkable reproducibility of egress, with an increased output when directly compared to human bone scaffolds over 28 days. Immunofluorescence demonstrated the persistence of CD34(+) cells within the scaffolds for the entirety of the culture. To characterise scaffold outputs, we designed a flow cytometry panel that utilises surface marker expression observed in standard 2D erythroid and megakaryocyte cultures. This showed that the egress population is comprised of haematopoietic progenitor cells (CD36(+)GPA(-/low)). Control cultures conducted in parallel but in the absence of a scaffold were also generally maintained for the longevity of the culture albeit with a higher level of cell death. The harvested scaffold egress can also be expanded and differentiated to the reticulocyte stage. In summary, PU scaffolds can behave as a subtractive compartmentalised culture system retaining and allowing maintenance of the seeded "CD34(+) cell" population despite this population decreasing in amount as the culture progresses, whilst also facilitating egress of increasingly differentiated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.