ArticlePlant molecular biology2016
WSL3, a component of the plastid-encoded plastid RNA polymerase, is essential for early chloroplast development in rice.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
- OsFeSOD3 Functions as an Enzymatic Component of the PEP Complex, Bifunctionally Regulating Chloroplastic ROS Metabolism and Chloroplast Biogenesis in Rice.Plant biotechnology journal · 2026Article
- Biological agents and plant growth regulator promote rice growth and regenerative capacity.BMC plant biology · 2025Article
- OsPRDA1 Interacts With OsFSD2 To Promote Chloroplast Development by Regulating Chloroplast Gene Expression in Rice.Rice (New York, N.Y.) · 2025Article
- PAP1 and PAP7 are required for association of plastid-encoded RNA polymerase with DNA.Plant molecular biology · 2024Article
- Improving Rice Quality by Regulating the Heading Dates of Rice Varieties without Yield Penalties.Plants (Basel, Switzerland) · 2024Article
- PEP-ASSOCIATED PROTEIN 3 regulates rice tiller formation and grain yield by controlling chloroplast biogenesis.Plant physiology · 2024Article
- Physiological, Cytological, and Transcriptomic Analysis of Magnesium Protoporphyrin IX Methyltransferase Mutant Reveal Complex Genetic Regulatory Network Linking Chlorophyll Synthesis and Chloroplast Development in Rice.Plants (Basel, Switzerland) · 2023Article
- PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize.International journal of molecular sciences · 2021Article
- white panicle2 encoding thioredoxin z, regulates plastid RNA editing by interacting with multiple organellar RNA editing factors in rice.The New phytologist · 2021Article
- Characterization and Fine Mapping of a Yellow-Virescent Gene Regulating Chlorophyll Biosynthesis and Early Stage Chloroplast Development inG3 (Bethesda, Md.) · 2020Article
- WSL6 encoding an Era-type GTP-binding protein is essential for chloroplast development in rice.Plant molecular biology · 2019Article
- Genetic analysis and fine mapping of a qualitative trait locus wpb1 for albino panicle branches in rice.PloS one · 2019Article
- White Leaf and Panicle 2, encoding a PEP-associated protein, is required for chloroplast biogenesis under heat stress in rice.Journal of experimental botany · 2017Article
- OsPPR6, a pentatricopeptide repeat protein involved in editing and splicing chloroplast RNA, is required for chloroplast biogenesis in rice.Plant molecular biology · 2017Article
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Authors and funding
19 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastid-encoded plastid RNA polymerase (PEP), a dominant RNA polymerase in mature chloroplasts, consists of core subunits and peripheral subunits. Despite the importance of the peripheral subunits in control of PEP activity it is unclear how they interact with one another to exert physiological effects on chloroplast development and plant growth, especially in rice. Here, we report a mutant, designated wsl3 that lacks a peripheral subunit in rice. We isolated the WSL3 gene encoding an essential peripheral subunit of rice PEP complex, OsPAP1/OspTAC3 by map-based cloning, and verified its function by complementation analysis. The wsl3 mutant showed a typical expression pattern of plastid-encoded genes, suggesting that PEP activity was impaired. Using immunofluorescent labeling and immunoblotting, we found that WSL3 was localized to the chloroplast and associated with the nucleoid. In addition, we demonstrated that WSL3 interacted with PEP subunits in Y2H, BiFC and pull-down experiments. Furthermore, a cpDNA IP assay revealed that WSL3 was associated with the PEP complex during the entire transcription process. We provide evidence suggesting that WSL3 is essential for early chloroplast development by interacting with subunits of the PEP complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.