ArticleProceedings of the National Academy of Sciences of the United States of America2016
Conserved methionine dictates substrate preference in Nramp-family divalent metal transporters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 98 citations in OpenAlex.
- Molecular insights into cadmium transport and micronutrient crosstalk in rice: Towards minimizing grain Cd.Journal of integrative plant biology · 2026Review
- Molecular Evolution and Mechanisms of Plants NRAMP Transporters in Response to Heavy Metal Stress.Plants (Basel, Switzerland) · 2026Review
- Fluorescence-Based Ion Transport Assays Using Proteoliposomes.Bio-protocol · 2026Article
- Determinants of metal import and specificity in a bacterial transporter.bioRxiv : the preprint server for biology · 2026Article
- Article
- Structural and functional basis of proton-independent transition metal import by a canonical bacterial Nramp transporter.bioRxiv : the preprint server for biology · 2026Article
- Systematic mining and characterization of metal transporter families regulating zinc homeostasis provide insights into metal homeostasis inFrontiers in plant science · 2026Article
- Structural basis for metal ion transport by the human SLC11 proteins DMT1 and NRAMP1.Nature communications · 2025Article
- Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Evolution, classification, and mechanisms of transport, activity regulation, and substrate specificity of ZIP metal transporters.Critical reviews in biochemistry and molecular biology · 2024Review
- They all rock: A systematic comparison of conformational movements in LeuT-fold transporters.Structure (London, England : 1993) · 2024Article
- Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites.bioRxiv : the preprint server for biology · 2024Article
- Genome-wide identification and evolutionary analysis of the NRAMP gene family in the AC genomes of Brassica species.BMC plant biology · 2024Article
- Engineering rice Nramp5 modifies cadmium and manganese uptake selectivity using yeast assay system.Frontiers in plant science · 2024Article
- Iron from the gut: the role of divalent metal transporter 1.Journal of clinical biochemistry and nutrition · 2024Article
- Amino Acid Residues of the Metal Transporter OsNRAMP5 Responsible for Cadmium Absorption in Rice.Plants (Basel, Switzerland) · 2023Article
- Article
- High-resolution structures with bound MneLife · 2023Article
- Coupling metal stable isotope compositions and X-ray absorption spectroscopy to study metal pathways in soil-plant systems: a mini review.Metallomics : integrated biometal science · 2023Review
- A weak allele of OsNRAMP5 for safer rice.Journal of experimental botany · 2022Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Natural resistance-associated macrophage protein (Nramp) family transporters catalyze uptake of essential divalent transition metals like iron and manganese. To discriminate against abundant competitors, the Nramp metal-binding site should favor softer transition metals, which interact either covalently or ionically with coordinating molecules, over hard calcium and magnesium, which interact mainly ionically. The metal-binding site contains an unusual, but conserved, methionine, and its sulfur coordinates transition metal substrates, suggesting a vital role in their transport. Using a bacterial Nramp model system, we show that, surprisingly, this conserved methionine is dispensable for transport of the physiological manganese substrate and similar divalents iron and cobalt, with several small amino acid replacements still enabling robust uptake. Moreover, the methionine sulfur's presence makes the toxic metal cadmium a preferred substrate. However, a methionine-to-alanine substitution enables transport of calcium and magnesium. Thus, the putative evolutionary pressure to maintain the Nramp metal-binding methionine likely exists because it-more effectively than any other amino acid-increases selectivity for low-abundance transition metal transport in the presence of high-abundance divalents like calcium and magnesium.
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