ArticleScientific reports2016
Characterization of non-olfactory GPCRs in human sperm with a focus on GPR18.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
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- Advances in cannabinoid receptors pharmacology: from receptor structural insights to ligand discovery.Acta pharmacologica Sinica · 2025Review
- Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus).BMC genomics · 2023Article
- Novel GPR18 Ligands in Rodent Pharmacological Tests: Effects on Mood, Pain, and Eating Disorders.International journal of molecular sciences · 2023Article
- N-arachidonylglycine is a caloric state-dependent circulating metabolite which regulates human CD4iScience · 2023Article
- G-Protein Coupled Receptors in Human Sperm: An In Silico Approach to Identify Potential Modulatory Targets.Molecules (Basel, Switzerland) · 2022Article
- Organ-specific host differential gene expression analysis in systemic candidiasis: A systems biology approach.Microbial pathogenesis · 2022Article
- Activation of PTH1R alleviates epididymitis and orchitis through Gq and β-arrestin-1 pathways.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
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- Potential genes and pathways associated with heterotopic ossification derived from analyses of gene expression profiles.Journal of orthopaedic surgery and research · 2021Article
- The Immunopathology of COVID-19 and the Cannabis Paradigm.Frontiers in immunology · 2021Review
- Therapeutic Exploitation of GPR18: Beyond the Cannabinoids?Journal of medicinal chemistry · 2020Review
- Discovery of Tricyclic Xanthines as Agonists of the Cannabinoid-Activated Orphan G-Protein-Coupled Receptor GPR18.ACS medicinal chemistry letters · 2020Article
- Knockout of mouse receptor accessory protein 6 leads to sperm function and morphology defects†.Biology of reproduction · 2020Article
- Synapsins are expressed at neuronal and non-neuronal locations in Octopus vulgaris.Scientific reports · 2019Article
- Uncovering sperm metabolome to discover biomarkers for bull fertility.BMC genomics · 2019Article
- GPCRomics: An Approach to Discover GPCR Drug Targets.Trends in pharmacological sciences · 2019Review
- Gamete-mediated mate choice: towards a more inclusive view of sexual selection.Proceedings. Biological sciences · 2018Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G protein-coupled receptors (GPCRs) transduce external chemical cues into intracellular signals and are involved in a plethora of physiological processes, but knowledge regarding the function of these receptors in spermatozoa is limited. In the present study, we performed RNA-Seq and analyzed the expression of the all GPCRs except olfactory receptors in human spermatozoa. We revealed the expression of up to 223 different GPCR transcripts in human spermatozoa (FPKM > 0.1) and identified GPR18, a newly described cannabinoid receptor, together with GPR137 and GPR135, as one of the three most highly expressed GPCRs. To date, the expression of GPR18 was completely unknown in human spermatozoa. We confirmed GPR18 expression using RT-PCR and immuncytochemistry experiments and localized the GPR18 protein in the midpiece of human spermatozoa. Stimulation of human spermatozoa with the GPR18 ligand N-arachidonoylglycine induced the phosphorylation of 12 protein kinases, some of them are for example known to be involved in the acrosome reaction. In line with this, N-arachidonoylglycine affected the cytoskeleton by changing levels of F-actin and inducing the acrosome reaction in human spermatozoa in a concentration-dependent manner. Our results indicate that GPR18 might be involved in physiological processes of human spermatozoa, suggesting GPR18 to be a potential player in sperm physiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.