ArticleDevelopment genes and evolution2016
C-terminal residues specific to Vasa among DEAD-box helicases are required for its functions in piRNA biogenesis and embryonic patterning.
Article in Development genes and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Conserved RNA helicase Vasa regulates ribonucleoprotein condensate dynamics and mRNA localization.iScience · 2026Article
- Developmental expression of Ap-Vas proteins in aphids and flies reveals their evolutionary roles in insects.Insect molecular biology · 2026Article
- Combinatorial tagging generates a multi-purpose knock-in mouse model revealing phase separation-dependent germ granules in RNA homeostasis and germline development.Cell death and differentiation · 2025Article
- Germ cell depletion using HSV-TK in mouse testes.Scientific reports · 2025Article
- RNA Helicase Vasa as a Multifunctional Conservative Regulator of Gametogenesis in Eukaryotes.Current issues in molecular biology · 2023Review
- Vasa, a regulator of localized mRNA translation on the spindle.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Review
- The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells.Communications biology · 2023Article
- GLH-1/Vasa represses neuropeptide expression and drives spermiogenesis in the C. elegans germline.Developmental biology · 2022Article
- Vasa nucleates asymmetric translation along the mitotic spindle during unequal cell divisions.Nature communications · 2022Article
- Building RNA-protein germ granules: insights from the multifaceted functions of DEAD-box helicase Vasa/Ddx4 in germline development.Cellular and molecular life sciences : CMLS · 2021Review
- Oogenesis of Hematophagous MidgeInsects · 2020Article
- Single nucleotide polymorphisms in piRNA-pathway genes: an insight into genetic determinants of human diseases.Molecular genetics and genomics : MGG · 2020Review
- Article
- Germ Cell Lineage Homeostasis inGenetics · 2019Article
- Wolbachia and host germline components compete for kinesin-mediated transport to the posterior pole of the Drosophila oocyte.PLoS pathogens · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DEAD-box RNA helicase Vasa (Vas, also known as DDX4) is required for germ cell development. In Drosophila, analysis of hypomorphic mutations has implicated maternally expressed Vas in germ cell formation and posterior embryonic patterning. vas-null females, which rarely complete oogenesis, exhibit defects in mitotic progression of germline stem cells, Piwi-interacting RNA (piRNA)-mediated transposon silencing, and translation of Gurken (Grk), an EGFR ligand. The carboxy-terminal region of Vas orthologs throughout the animal kingdom consists of several acidic residues as well as an invariant tryptophan in the penultimate or ultimate position (Trp660 in Drosophila melanogaster). Using CRISPR/Cas9 gene editing, we made a substitution mutant in this residue. Replacing Trp660 by Glu (W660E) abolishes the ability of Vas to support germ cell formation and embryonic patterning and greatly reduces Vas activity in piRNA biogenesis, as measured by transposon silencing, and in activating Grk translation. A conservative substitution (W660F) has much milder phenotypic consequences. In addition, females expressing only a form of Vas in which the seven C-terminal amino acids were replaced with the corresponding residues from Belle (Bel, also known as DDX3) show defects in perinuclear nuage assembly and transposon silencing. Oogenesis in females expressing only the chimeric Vas arrests early; however, in a vas
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