Evidence map›Paper›PMID 27572841›Full record

ArticleNature microbiology2016

Selection of antigenically advanced variants of seasonal influenza viruses.

Chengjun Li, Masato Hatta, David F Burke, Jihui Ping, Ying Zhang, Makoto Ozawa, Andrew S Taft, Subash C Das, Anthony P Hanson, Jiasheng Song and 24 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. Article
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  3. Molecular Surveillance, Evolution, and Vaccine Strain Match of theInternational journal of molecular sciences · 2026
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  6. PA and PA-X: two key proteins from segment 3 of the influenza viruses.Frontiers in cellular and infection microbiology · 2025
    Review
  7. Article
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  11. Article
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  14. Article
  15. Article
  16. Antigenic characterization of influenza and SARS-CoV-2 viruses.Analytical and bioanalytical chemistry · 2022
    Review
  17. Article
  18. Article
  19. Article
  20. mBio · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

34 authors.

Chengjun Li *Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Masato Hatta *Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
David F Burke *Department of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
Jihui Ping *Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Ying Zhang *Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Makoto OzawaInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Andrew S TaftInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Subash C DasInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Anthony P HansonInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Jiasheng SongInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Masaki ImaiInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Peter R WilkerInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Tokiko WatanabeERATO Infection-Induced Host Responses Project, Saitama 332-0012, Japan.
Shinji WatanabeERATO Infection-Induced Host Responses Project, Saitama 332-0012, Japan.
Mutsumi ItoDivision of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
Kiyoko Iwatsuki-HorimotoDivision of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
Colin A RussellWorld Health Organization Collaborating Centre for Modeling, Evolution, and Control of Emerging Infectious Diseases, Cambridge CB2 3EJ, UK.
Sarah L JamesDepartment of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
Eugene SkepnerDepartment of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
Eileen A MaherInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Gabriele NeumannInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.
Alexander I KlimovInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA.
Anne KelsoWHO Collaborating Centre for Reference and Research on Influenza (VIDRL) at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
John McCauleyDivision of Virology, MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom.
Dayan WangChinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Yuelong ShuChinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Takato OdagiriInfluenza Virus Research Center, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan.
Masato TashiroInfluenza Virus Research Center, National Institute of Infectious Diseases, Musashi-Murayama, Tokyo 208-0011, Japan.
Xiyan XuInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA.
David E WentworthInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA.
Jacqueline M KatzInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA.
Nancy J CoxInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30033, USA.
Derek J SmithDepartment of Zoology, University of Cambridge, Cambridge CB2 3EJ, UK.
Yoshihiro KawaokaInfluenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53711, USA.

Funding

Virology Training ProgramT32AI078985 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI LAMBERT, PAUL F. · 2009 to 2018
$3.3M
NIH Director's Pioneer Award:Antigenic Cartography (RMI)DP1OD000490 · OD · UNIVERSITY OF CAMBRIDGE · PI SMITH, DEREK JAMES · 2005 to 2009
$2.6M
Medical Research Council MC_U117512723NIAID NIH HHS HHSN266200700010CNIAID NIH HHS HHSN272201400008CNIAID NIH HHS T32 AI078985NIH HHS DP1 OD000490Wellcome TrustWellcome Trust 087982AIA
6 · The paper itself

Abstract

Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. We also selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014-2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature.

Indexed as

Antigenic VariationAmino Acid SubstitutionAnimalsAntigens, ViralEvolution, MolecularFerretsHemagglutinin Glycoproteins, Influenza VirusHumansImmune EvasionInfluenza A Virus, H1N1 SubtypeInfluenza A Virus, H3N2 SubtypeInfluenza, HumanInfluenza VaccinesMiceOrthomyxoviridae InfectionsSeasonsAntigens, ViralHemagglutinin Glycoproteins, Influenza VirusInfluenza Vaccines

Identifiers

PMID27572841
PMCPMC5087998

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.