Evidence map›Paper›PMID 27572735›Full record

ReviewMethods in enzymology2016

Databases and Archiving for CryoEM.

A Patwardhan, C L Lawson

Abstract readReview
In one paragraph

Review in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed.

  1. Article
  2. Article
  3. Cryo-EM structure of the folded-back state of human β-cardiac myosin.bioRxiv : the preprint server for biology · 2023
    Article
  4. Review
  5. New system for archiving integrative structures.Acta crystallographica. Section D, Structural biology · 2021
    Article
  6. Multi-body Refinement of Cryo-EM Images in RELION.Methods in molecular biology (Clifton, N.J.) · 2021
    Article
  7. Could Egg White Lysozyme be Solved by Single Particle Cryo-EM?Journal of chemical information and modeling · 2020
    Article
  8. Reporting on the future of integrative structural biology ORAU workshop.Frontiers in bioscience (Landmark edition) · 2020
    Article
  9. Evolving data standards for cryo-EM structures.Structural dynamics (Melville, N.Y.) · 2020
    Article
  10. Article
  11. Reducing cryoEM file storage using lossy image formats.Journal of structural biology · 2019
    Article
  12. Article
  13. Article
  14. Comparing cryo-EM structures.Journal of structural biology · 2018
    Article
  15. Review
  16. Article
  17. Article
  18. Review
  19. EMDB Web Resources.Current protocols in bioinformatics · 2018
    Article
  20. Trends in the Electron Microscopy Data Bank (EMDB).Acta crystallographica. Section D, Structural biology · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

A PatwardhanProtein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, United Kingdom. Electronic address: ardan@ebi.ac.uk.
C L LawsonResearch Collaboratory for Structural Bioinformatics, Center for Integrative Proteomics Research, Rutgers, The State University of New Jersey, Piscataway, NJ, United States. Electronic address: cathy.lawson@rutgers.edu.

Funding

Unified Data Resource for Large Complexes Determined by Cryo-Electron MicroscopyR01GM079429 · NIGMS · STANFORD UNIVERSITY · PI CHIU, WAH · 2007 to 2021
$11.6M
Biotechnology and Biological Sciences Research Council BB/G022577/1Biotechnology and Biological Sciences Research Council BB/M018423/1Medical Research Council MR/L007835NIGMS NIH HHS R01 GM079429Wellcome TrustWellcome Trust 088944Wellcome Trust 104948
6 · The paper itself

Abstract

CryoEM in structural biology is currently served by three public archives-EMDB for 3DEM reconstructions, PDB for models built from 3DEM reconstructions, and EMPIAR for the raw 2D image data used to obtain the 3DEM reconstructions. These archives play a vital role for both the structural community and the wider biological community in making the data accessible so that results may be reused, reassessed, and integrated with other structural and bioinformatics resources. The important role of the archives is underpinned by the fact that many journals mandate the deposition of data to PDB and EMDB on publication. The field is currently undergoing transformative changes where on the one hand high-resolution structures are becoming a routine occurrence while on the other hand electron tomography is enabling the study of macromolecules in the cellular context. Concomitantly the archives are evolving to best serve their stakeholder communities. In this chapter, we describe the current state of the archives, resources available for depositing, accessing, searching, visualizing and validating data, on-going community-wide initiatives and opportunities, and challenges for the future.

Indexed as

SoftwareComputational BiologyCryoelectron MicroscopyDatabases as TopicDatabases, ProteinElectron Microscope TomographyInformation DisseminationModels, MolecularProteinsProteinsDatabasesEMDataBankEMDBEMPIARProtein Data BankValidation

Identifiers

PMID27572735
PMCPMC5351295

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.