ReviewMethods in enzymology2016
Single-Particle Refinement and Variability Analysis in EMAN2.1.
Review in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed.
- Molecular mechanism of IF1- and IF2-driven translation initiation in bacteria.bioRxiv : the preprint server for biology · 2026Article
- Cryo-EM structures of HBV capsids from human cells at near-atomic resolution.Structure (London, England : 1993) · 2026Article
- Structural polymorphism of two-dimensional lattices assembled from baculoviral capsid proteins.Virologica Sinica · 2025Article
- In situ structure and assembly of the ABC-type tripartite pump MacAB-TolC.Communications biology · 2025Article
- Amphipathic Antimicrobial Peptides Illuminate a Reciprocal Relationship Between Self-assembly and Cytolytic Activity.Angewandte Chemie (International ed. in English) · 2025Article
- Genetically Engineered, Multichromophore Virus-Like Nanoparticles with Ultranarrow Distribution of Emission Intensity.ACS nano · 2025Article
- Cryo-EM structures of Banna virus in multiple states reveal stepwise detachment of viral spikes.Nature communications · 2024Article
- Structural Analysis of Protein Complexes by Cryo-Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Structural Analysis of Protein Complexes by Cryo-Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Review
- HIV-1 Vpu protein forms stable oligomers in aqueous solution via its transmembrane domain self-association.Scientific reports · 2023Article
- HIV-1 Vpu protein forms stable oligomers in aqueous solution via its transmembrane domain self-association.bioRxiv : the preprint server for biology · 2023Article
- Direct observation of the conformational states of formin mDia1 at actin filament barbed ends and along the filament.Molecular biology of the cell · 2023Article
- Continuum dynamics and statistical correction of compositional heterogeneity in multivalent IDP oligomers resolved by single-particle EM.Journal of molecular biology · 2022Article
- Quantitative mining of compositional heterogeneity in cryo-EM datasets of ribosome assembly intermediates.Structure (London, England : 1993) · 2022Article
- Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 Å in cells.Nature methods · 2021Article
- Virus Assembly Pathways: Straying Away but Not Too Far.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Connexin-46/50 in a dynamic lipid environment resolved by CryoEM at 1.9 Å.Nature communications · 2020Article
- Computational Methods for Single-Particle Electron Cryomicroscopy.Annual review of biomedical data science · 2020Review
- Structure of the host cell recognition and penetration machinery of a Staphylococcus aureus bacteriophage.PLoS pathogens · 2020Article
- Structure of a rabies virus polymerase complex from electron cryo-microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
CryoEM single-particle reconstruction has been growing rapidly over the last 3 years largely due to the development of direct electron detectors, which have provided data with dramatic improvements in image quality. It is now possible in many cases to produce near-atomic resolution structures, and yet 2/3 of published structures remain at substantially lower resolutions. One important cause for this is compositional and conformational heterogeneity, which is both a resolution-limiting factor and presenting a unique opportunity to better relate structure to function. This manuscript discusses the canonical methods for high-resolution refinement in EMAN2.12, and then considers the wide range of available methods within this package for resolving structural variability, targeting both improved resolution and additional knowledge about particle dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.