ArticleScientific reports2016
In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- A systematic review of the research progress of non-coding RNA in neuroinflammation and immune regulation in cerebral infarction/ischemia-reperfusion injury.Frontiers in immunology · 2022Pooled it
- Combination of Cas9 and adeno-associated vectors enables efficient in vivo knockdown of precise miRNAs in the rodent and primate brain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Epigenetics-targeted drugs: current paradigms and future challenges.Signal transduction and targeted therapy · 2024Review
- Akt is a mediator of artery specification during zebrafish development.Development (Cambridge, England) · 2024Article
- Review
- Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium.Nature cell biology · 2023Article
- Targeting miRNA by CRISPR/Cas in cancer: advantages and challenges.Military Medical Research · 2023Review
- MicroRNAs in the Mouse Developing Retina.International journal of molecular sciences · 2023Review
- Cancer Detection Using an Artificial Secretable MicroRNA Found in Blood and Urine.Biomedicines · 2022Article
- Dyrk1b promotes autophagy during skeletal muscle differentiation by upregulating 4e-bp1.Cellular signalling · 2022Article
- Emerging roles of noncoding micro RNAs and circular RNAs in bovine mastitis: Regulation, breeding, diagnosis, and therapy.Frontiers in microbiology · 2022Review
- miRNA- and lncRNA-Based Therapeutics for Non-Hodgkin's Lymphoma: Moving towards an RNA-Guided Precision Medicine.Cancers · 2021Review
- PPIL4 is essential for brain angiogenesis and implicated in intracranial aneurysms in humans.Nature medicine · 2021Article
- The roles of microRNAs in mouse development.Nature reviews. Genetics · 2021Review
- MicroRNA26 attenuates vascular smooth muscle maturation via endothelial BMP signalling.PLoS genetics · 2019Article
- MicroRNA-dependent regulation of biomechanical genes establishes tissue stiffness homeostasis.Nature cell biology · 2019Article
- High Efficiency Targeting of Non-coding Sequences Using CRISPR/Cas9 System in Tilapia.G3 (Bethesda, Md.) · 2019Article
- Review
- CRISPR/Cas9-mediated noncoding RNA editing in human cancers.RNA biology · 2018Review
- Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System.Stem cells international · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
A large number of microRNAs (miRNAs) are grouped into families derived from the same phylogenetic ancestors. miRNAs within a family often share the same physiological functions despite differences in their primary sequences, secondary structures, or chromosomal locations. Consequently, the generation of animal models to analyze the activity of miRNA families is extremely challenging. Using zebrafish as a model system, we successfully provide experimental evidence that a large number of miRNAs can be simultaneously mutated to abrogate the activity of an entire miRNA family. We show that injection of the Cas9 nuclease and two, four, ten, and up to twenty-four multiplexed single guide RNAs (sgRNAs) can induce mutations in 90% of the miRNA genomic sequences analyzed. We performed a survey of these 45 mutations in 10 miRNA genes, analyzing the impact of our mutagenesis strategy on the processing of each miRNA both computationally and in vivo. Our results offer an effective approach to mutate and study the activity of miRNA families and pave the way for further analysis on the function of complex miRNA families in higher multicellular organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.